The article presents the results of in vitro studies on cytotoxicity and antibacterial activity of new MTA-type cements, developed on the basis of the sintered tricalcium silicate enriched with ZnO, along with an agent introducing the radiopacity in the form of ZrO₂. The new materials have been developed to ensure that their physical and chemical properties are suited for endodontic applications. The cements were evaluated via characterisation of setting time, compressive strength, as well as translucency on X-ray images, and bioactivity in the simulated body fluid (SBF). The μCT was used to test the influence of the ZrO₂ grains in the powder component on the microstructure of the produced cement. Then, the cytotoxic action of the cements was evaluated by applying a reference L-929 cell line. The conditions of the culture upon contact with the tested materials or with extracts from the cements were assessed using image analysis or an MTT colorimetric assay. Two strains of streptococci, Streptococcus mutans and Streptococcus sanguinis, were used to study the antibacterial activity of the tested cements with ZrO₂ acting as the agent introducing the radiopacity. The new cements are characterised by appropriate properties as far as retrograde root canal filling is concerned.

中文翻译：

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ZrO2 引入的具有不透射线的三氧化二矿物骨料水泥的细胞毒性和抗菌活性

本文介绍了新型 MTA 型水泥的细胞毒性和抗菌活性的体外研究结果，该水泥是在富含 ZnO 的烧结硅酸三钙和以 ZrO ₂形式引入射线不透性的试剂的基础上开发的。新材料的开发旨在确保其物理和化学特性适用于牙髓应用。通过对凝固时间、抗压强度以及 X 射线图像的半透明性和模拟体液 (SBF) 中的生物活性的表征来评估水泥。μ CT用于测试ZrO ₂的影响粉末成分中的晶粒对所生产水泥的微观结构的影响。然后，通过应用参考 L-929 细胞系评估水泥的细胞毒性作用。使用图像分析或 MTT 比色测定法评估与测试材料或水泥提取物接触后的培养条件。使用两种链球菌菌株，变形链球菌和血链球菌，以ZrO ₂作为引入射线不透性的试剂，研究测试水泥的抗菌活性。就逆行根管充填而言，新水泥具有适当的特性。