BACKGROUND:Morin, (3,5,7,2′,4′-pentahydroxyflavone), is a polyphenolic compound belonging to bio-flavonoids and is predominantly isolated from the family Moraceae. Previously, studies demonstrated the health benefits of morin using human and animal models. Despite its importance as a bioactive compound, the genetic architecture of the morin biosynthetic pathway is still unclear. OBJECTIVE:To understand the genetic architecture of the morin biosynthetic pathway, the following components were analyzed: (1) cis-responsive element (CRE)-mediated regulation, (2) microRNAs (miRNA)-mediated post-transcriptional silencing, and (3) tissue-specific in silico gene expression. METHODS:To understand the genetic architecture of morin biosynthetic pathway, in silico survey was carried out using different web servers (MorusDB, MEME suite, NCBI database, PlantCARE, and psRNATarget) and collected mRNA, protein sequences, and expressed microarray data. TBtools was employed for depicting protein and promoter motifs and the heatmap preparation of tissue-specific expression of genes involved in the morin biosynthesis. RESULTS:The current data mining study highlighted the morin biosynthetic pathway associated genes, namely, phenylalanine ammonia-lyase (MnPAL), chalcone synthase A (MnCSA), chalcone-flavonone isomerase (MnCFI), and flavonoid 3′,5′-hydroxylase (MnFH) are transcriptionally regulated by different growth, development, and stress-responsive CREs. Differential expression profiles how MnPAL (L484_024373) and MnCFI (L484_011241) genes were upregulated across selected tissues. Moreover, miRNA-mediated post-transcriptional silencing was identified. CONCLUSIONS:This study will improve our understanding of morin biosynthesis, thus helping improve production via metabolic engineering.

中文翻译：

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桑椹 (Morus notabilis) 桑椹 (五羟基黄酮) 生物合成途径的遗传结构：一种计算机方法

背景：桑葚（3,5,7,2',4'-五羟基黄酮）是一种多酚类化合物，属于生物类黄酮，主要从桑科植物中分离得到。此前，研究通过人类和动物模型证明了莫林对健康的益处。尽管它作为一种生物活性化合物很重要，但桑色素生物合成途径的遗传结构仍不清楚。目的：为了了解桑色素生物合成途径的遗传结构，分析了以下成分：（1）顺式反应元件（CRE）介导的调节，（2）微小RNA（miRNA）介导的转录后沉默，以及（3） ) 组织特异性计算机基因表达。方法：为了解桑叶素生物合成途径的遗传结构，使用不同的网络服务器（MorusDB、MEME 套件、NCBI 数据库、PlantCARE、和 psRNATarget）并收集 mRNA、蛋白质序列和表达的微阵列数据。TBtools 被用于描绘蛋白质和启动子基序，以及参与桑黄素生物合成的基因的组织特异性表达的热图制备。结果：目前的数据挖掘研究突出了桑黄素生物合成途径相关基因，即苯丙氨酸解氨酶（MnPAL）、查尔酮合酶A（MnCSA）、查尔酮黄酮异构酶（MnCFI）和类黄酮3',5'-羟化酶。 MnFH) 受不同的生长、发育和应激反应 CRE 的转录调控。差异表达描述了 MnPAL (L484_024373) 和 MnCFI (L484_011241) 基因如何在选定组织中上调。此外，鉴定了 miRNA 介导的转录后沉默。结论：