In this study, the correlation analysis between gonadosomatic index and growth traits of M. nipponense was carried out for the first time. The results proved that short sexual maturity M. nipponense had a significant negatively effect on the female prawn size (p < 0.01) but had almost no effect on the male (p > 0.05). The full-length Vitellogenin gene of M. nipponense (Mn-Vg) were applied to detect polymorphism and the correlation between SNP and ovarian development. The total length of Mn-Vg was 10,038 bp, including 14 exons and 13 introns. 31 candidate SNP loci of Mn-Vg were identified and the genetic diversity of these loci in the M. nipponense population was moderate polymorphism. The correlation between Mn-Vg SNPs and ovarian development was further investigated. 6 SNPs were significantly correlated with female GSI. The genotype CC in C+948A, GG in G+1209A, AG in A+1879G, TT and AT in T+4458A, AT in A+7080A and AA in A+9435G are significantly associated with rapid ovary maturation. Further statistical analysis results between SNPs and female growth index showed five SNP loci were significantly associated with female growth traits. Three of them significantly associated with ovarian maturation (C+948A, T+4458A and A+9435G). By comparison, genotype TT in T+4458A was significantly positively correlated with both ovary development and growth traits. The results will provide a basis for marker-assisted breeding of M. nipponense, as well as sustainable development of aquaculture and large-scale breeding.

中文翻译：

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鉴定卵黄蛋白原基因中的SNP及其与日本沼虾卵巢发育和生长的关系

本研究首次开展了性腺体指数与日本沼虾生长性状的相关性分析。结果证明，性成熟期短的日本对虾对雌虾体型有显着的负面影响（p  < 0.01），但对雄虾几乎没有影响（p  > 0.05）。应用M. nipponense ( Mn-Vg )全长卵黄原蛋白基因检测多态性及SNP与卵巢发育的相关性。Mn-Vg全长10038bp，包括14个外显子和13个内含子。Mn-Vg的 31 个候选 SNP 位点被鉴定并且这些基因座在M. nipponense群体中的遗传多样性是中度多态性。Mn-Vg的相关性进一步研究了 SNP 和卵巢发育。6个SNP与女性GSI显着相关。C+948A中的CC基因型、G+1209A中的GG基因型、A+1879G中的AG基因型、T+4458A中的TT和AT基因型、A+7080A中的AT基因型和A+9435G中的AA基因型与卵巢快速成熟显着相关。SNPs与雌性生长指数的进一步统计分析结果表明，5个SNP位点与雌性生长性状显着相关。其中三个与卵巢成熟显着相关（C+948A、T+4458A 和 A+9435G）。相比之下，T+4458A基因型TT与卵巢发育和生长性状均呈显着正相关。研究结果将为日本沼虾的分子标记辅助育种，以及水产养殖和规模化养殖的可持续发展提供依据。