Protein adsorption has a crucial effect on biocompatibility during the interaction of biomaterial surfaces and the biological environment. It is significant to understand and control the interactions among biomaterials and proteins for several biomedical applications. Surface engineering plays a significant role in determining biocompatibility by tuning the effects directly on proteins. In this study, amino acid (histidine and leucine) conjugated self-assembled molecules were synthesized and used to modify silicon dioxide (SiO₂) surfaces to investigate protein adsorption behavior. Silicon dioxide surfaces were modified with (3-aminopropyl)triethoxysilane-conjugated histidine and leucine amino acids. Modified silicon dioxide surfaces were characterized by water contact angle measurements and X-ray photoelectron spectroscopy analysis. Protein adsorption (human serum albumin, fibrinogen and immunoglobulin G) on silicon dioxide-coated crystals was investigated in situ by using a quartz crystal microbalance (QCM) biosensor. From the results, model proteins showed different selectivities to the amino-acid-conjugated silicon dioxide-coated crystals depending on the type of the amino acid and concentration. Consequently, this controlled chemistry on the surface of biomaterials has a great potential to manipulate protein adsorption and enhance the biocompatibility of biomaterials for various biomedical applications.

中文翻译：

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氨基酸共轭自组装分子修饰的 SiO2 表面上的蛋白质吸附

在生物材料表面和生物环境相互作用的过程中，蛋白质吸附对生物相容性具有至关重要的影响。对于几种生物医学应用而言，了解和控制生物材料和蛋白质之间的相互作用具有重要意义。表面工程通过直接调整对蛋白质的影响，在确定生物相容性方面发挥着重要作用。本研究合成了氨基酸（组氨酸和亮氨酸）缀合的自组装分子，并用于修饰二氧化硅（SiO ₂) 表面来研究蛋白质吸附行为。二氧化硅表面用 (3-氨基丙基) 三乙氧基硅烷共轭组氨酸和亮氨酸氨基酸修饰。通过水接触角测量和 X 射线光电子能谱分析对改性二氧化硅表面进行了表征。使用石英晶体微量天平 (QCM) 生物传感器原位研究了二氧化硅涂层晶体上的蛋白质吸附（人血清白蛋白、纤维蛋白原和免疫球蛋白 G）。从结果来看，模型蛋白对氨基酸共轭二氧化硅包被的晶体表现出不同的选择性，这取决于氨基酸的类型和浓度。最后，