ABSTRACT

We recently reported shrinkage of untreatable recurrent glioblastoma (GBM) in an end-stage patient using noninvasive brain stimulation with a spinning oscillating magnetic field (sOMF)-generating device called the Oncomagnetic device. Our in vitro experiments demonstrated selective cancer cell death while sparing normal cells by sOMF-induced increase in intracellular reactive oxygen species (ROS) levels due to magnetic perturbation of mitochondrial electron transport. Here, we describe the results of an in vivo study assessing the toxicity of chronic sOMF stimulation in mice using a newly constructed apparatus comprised of the sOMF-generating active components of the Oncomagnetic device. We chronically stimulated 10 normal 60-day old female C57BL/6 mice in their housing cages for 2 h 3 times a day, as in the patient treatment protocol, over 4 months. We also studied the effects of 2-h acute sOMF stimulation. Our observations and those of blinded independent veterinary staff observers, indicated no significant adverse effects of chronic or acute sOMF stimulation on the health, behavior, electrocardiographic and electroencephalographic activities, hematologic profile, and brain and other tissue and organ morphology of treated mice compared to age-matched untreated control mice. These findings suggest that short- and long-term therapies with the Oncomagnetic device are safe and well tolerated.

摘要

我们最近报道了使用非侵入性脑刺激和旋转振荡磁场 (sOMF) 产生装置称为 Oncomagnetic 装置的终末期患者无法治疗的复发性胶质母细胞瘤 (GBM) 缩小。我们的体外实验表明，由于线粒体电子传递的磁扰动，sOMF 诱导的细胞内活性氧 (ROS) 水平增加，因此可以选择性地杀死癌细胞，同时保留正常细胞。在这里，我们描述了体内的结果研究使用由 Oncomagnetic 装置的产生 sOMF 的活性成分组成的新型装置评估慢性 sOMF 刺激对小鼠的毒性。我们长期刺激 10 只正常的 60 天大的雌性 C57BL/6 小鼠在它们的笼子里，每天 3 次，每次 2 小时，如患者治疗方案，超过 4 个月。我们还研究了 2 小时急性 sOMF 刺激的影响。我们的观察和不知情的独立兽医观察员的观察表明，与年龄相比，慢性或急性 sOMF 刺激对接受治疗的小鼠的健康、行为、心电图和脑电图活动、血液学特征以及大脑和其他组织和器官形态没有显着的不利影响-匹配的未处理对照小鼠。