This research aimed to study the therapeutic potential of Viburnum opulus L. (VO) powder extracts on human breast cancer and normal breast epithelial cell lines. The half-maximum inhibitory concentration IC50 value was 51.32 μg/mL for 24 h using an MTT assay. The total apoptotic induction was found to be 40.50% for MCF-7 cells exposed to 100 μg/mL VO powder extract for 48 h. The genotoxic effect in MCF-7 cells was statistically significantly increased in a dose-dependent manner after exposure to VO powder extracts. P1 class glutathione-S-transferase (GSTP1) expression level was noticed protective feature in drug resistance metabolism. GSTP1 activities were found statistically significant differences between all of the groups (**P <0.01). GSTP1 overexpressed in MCF-7 control cells and expression levels decreased in the MCF-7 groups that were treated with VO powder extract at different concentrations of 25, 50 and 100 μg/mL for 24, 48 and 72 h. These results suggest that VO powder extracts suppress the proliferation and GSTP1 expression of MCF-7 cells and this suppression is attributed to both induction of apoptosis and DNA damage. This study provided the information that VO powder extract may be suitable for drug therapy efficacy at the molecular level.

中文翻译：

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蔓越莓对 MCF-7 人乳腺癌细胞的评估

本研究旨在研究 Viburnum opulus L. (VO) 粉末提取物对人乳腺癌和正常乳腺上皮细胞系的治疗潜力。使用 MTT 法测定 24 h 的半数最大抑制浓度 IC50 值为 51.32 μg/mL。发现暴露于 100 μg/mL VO 粉末提取物 48 小时的 MCF-7 细胞的总凋亡诱导率为 40.50%。暴露于 VO 粉末提取物后，MCF-7 细胞中的基因毒性作用以剂量依赖性方式显着增加。P1类谷胱甘肽-S-转移酶（GSTP1）表达水平在耐药性代谢中具有保护作用。GSTP1 活性在所有组之间存在统计学显着差异（**P <0.01）。GSTP1 在 MCF-7 对照细胞中过表达，而 MCF-7 组的表达水平在用 25、50 和 100 μg/mL 不同浓度的 VO2 粉末提取物处理 24、48 和 72 小时后降低。这些结果表明，VO 粉末提取物抑制 MCF-7 细胞的增殖和 GSTP1 表达，这种抑制归因于细胞凋亡和 DNA 损伤的诱导。该研究提供了VO粉末提取物在分子水平上可能适用于药物治疗功效的信息。