ABSTRACT

Introduction

Riella Mont. is unusual among bryophyte genera because its species have an ephemeral habit and grow submerged in fresh or brackish waters. Most species show fragmented distribution areas and have few known populations and are thus of conservation concern. The flagship Mediterranean species R. helicophylla (Bory & Mont.) Mont. has recently been taxonomically reassessed, and most populations have been placed in a new species, R. macrocarpa (P.Allorge) Puche, Segarra-Moragues, Sabovlj., M.Infante & Heras. Here we describe the development of nuclear microsatellite markers for R. macrocarpa with the aim of characterising the population genetic dynamics of this species of conservation concern, and we report their potential use in other species of the genus.

Methods

Nuclear microsatellite markers were isolated from 454 genomic libraries and amplification parameters optimised for the set of loci finally selected.

Key results and conclusions

Nine microsatellite loci were characterised and amplified a total of 53 alleles in a sample of 120 individuals from four populations, having a mean of 5.89 alleles per locus. Unbiased genetic diversity for the polymorphic loci within populations ranged from 0.067 to 0.791. Cross-species transferability assayed in six other species of Riella covering reproductive and taxonomic diversity in the genus showed transferability success rates ranging from 77.8% to 100%, with 33.3% to 57.1% of the successfully transferred loci being polymorphic within species. These markers will be useful for studying population genetic diversity and structure and will contribute towards better conservation management of populations of R. macrocarpa and other species of Riella.

中文翻译：

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Riella macrocarpa (P.Allorge) Puche 等人的微卫星标记的分离、表征和跨物种可转移性。(Riellaceae, Sphaerocarpales)

摘要

介绍

里埃拉山 在苔藓植物属中是不寻常的，因为它的物种具有短暂的习性，并在淡水或微咸水中生长。大多数物种的分布区域分散，已知种群很少，因此受到保护。地中海旗舰物种R. helicophylla (Bory & Mont.) Mont. 最近对分类进行了重新评估，大多数种群已被置于一个新物种中，R. macrocarpa (P.Allorge) Puche, Segarra-Moragues, Sabovlj., M.Infante & Heras。在这里，我们描述了R. macrocarpa核微卫星标记的发展为了描述这种受保护物种的种群遗传动态，我们报告了它们在该属其他物种中的潜在用途。

方法

从 454 个基因组文库中分离出核微卫星标记，并针对最终选择的基因座集优化扩增参数。

主要结果和结论

在来自四个群体的 120 个个体的样本中，对九个微卫星基因座进行了表征和扩增，总共有 53 个等位基因，每个基因座平均有 5.89 个等位基因。群体内多态位点的无偏遗传多样性范围为 0.067 至 0.791。在涵盖该属生殖和分类多样性的其他六种Riella中进行的跨物种可转移性分析显示可转移成功率从 77.8% 到 100% 不等，其中 33.3% 到 57.1% 的成功转移基因座在物种内是多态的。这些标记将有助于研究种群遗传多样性和结构，并将有助于更好地保护大果树和其他 Riella 物种的种群。