Glutaraldehyde (Glut)-crosslinked porcine pericardium and bovine pericardium are mainly consisted of collagen and widely used for the preparation of heterogenous bioprosthetic heart valves (BHV), which play an important role in the replacement therapy of severe valvular heart disease, while their durability is limited by degeneration due to calcification, thrombus, endothelialization difficulty and prosthetic valve endocarditis. Herein, we develop a novel BHV, namely, TPly-BP, based on natural tannic acid and polylysine to improve the durability of Glut crosslinked bovine pericardium (Glut-BP). Impressively, tannic acid and polylysine could form nanoaggregates via multiple hydrogen bonds and covalent bonds, and the introduction of nanoaggregates not only improved the mechanical properties and collagen stability but also endowed TPly-BP with good biocompatibility and hemocompatibility. Compared to Glut-BP, TPly-BP showed significantly reduced cytotoxicity, improved endothelial cell adhesion, a low hemolysis ratio and obviously reduced platelet adhesion. Importantly, TPly-BP exhibited great antibacterial and in vivo anti-calcification ability, which was expected to improve the in vivo durability of BHVs. These results suggested that TPly-BP would be a potential candidate for BHV.

中文翻译：

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基于多酚的混合纳米聚集体改性生物瓣叶的胶原纤维，以实现增强的机械、抗凝和抗钙化性能

戊二醛（Glut）交联的猪心包和牛心包主要由胶原蛋白组成，广泛用于异质生物人工心脏瓣膜（BHV）的制备，在重症瓣膜性心脏病的替代治疗中发挥着重要作用，同时其耐用性受限于钙化、血栓、内皮化困难和人工瓣膜心内膜炎引起的变性。在此，我们开发了一种基于天然单宁酸和聚赖氨酸的新型 BHV，即 TPly-BP，以提高 Glut 交联牛心包 (Glut-BP) 的耐久性。令人印象深刻的是，单宁酸和聚赖氨酸可以通过多个氢键和共价键形成纳米聚集体，并且纳米聚集体的引入不仅提高了力学性能和胶原蛋白的稳定性，而且赋予了TPly-BP良好的生物相容性和血液相容性。与 Glut-BP 相比，TPly-BP 的细胞毒性显着降低，内皮细胞粘附性改善，溶血率低，血小板粘附性明显降低。重要的是，TPly-BP 表现出出色的抗菌和体内抗钙化能力，有望提高 BHV 的体内耐久性。这些结果表明 TPly-BP 将成为 BHV 的潜在候选者。TPly-BP表现出很强的抗菌和体内抗钙化能力，有望提高BHV的体内耐久性。这些结果表明 TPly-BP 将成为 BHV 的潜在候选者。TPly-BP表现出很强的抗菌和体内抗钙化能力，有望提高BHV的体内耐久性。这些结果表明 TPly-BP 将成为 BHV 的潜在候选者。