The Dalbergieae lectin group encompasses several lectins with significant differences in their carbohydrate specificities and biological properties. The current work reports on the purification and characterization of a GalNAc/Gal-specific lectin from Vataireopsis araroba (Aguiar) Ducke, designated as VaL. The lectin was purified from the seeds in a single step using guar gum affinity chromatography. The lectin migrated as a single band of about 35 kDa on SDS-PAGE and, in native conditions, occurs as a homodimer. The purified lectin is stable at temperatures up to 60 °C and in a pH range from 7 to 8 and requires divalent cations for its activity. Sugar-inhibition assays demonstrate the lectin specificity towards N-acetyl-D-galactosamine, D-galactose and related sugars. Furthermore, glycan array analyses show that VaL interacts preferentially with glycans containing terminal GalNAc/Galβ1-4GlcNAc. Biological activity assays were performed using three insect cell lines: CF1 midgut cells from the spruce budworm Choristoneura fumiferana, S2 embryo cells from the fruit fly Drosophila melanogaster, and GutAW midgut cells from the corn earworm Helicoverpa zea. In vitro assays indicated a biostatic effect for VaL on CF1 cells, but not on S2 and GutAW cells. The lectin presented a biostatic effect by reducing the cell growth and inducing cell agglutination, suggesting an interaction with glycans on the cell surface. VaL has been characterized as a galactoside-specific lectin of the Dalbergieae tribe, with sequence similarity to lectins from Vatairea and Arachis.

黄檀科凝集素组包括几种在碳水化合物特异性和生物学特性方面存在显着差异的凝集素。目前的工作报告了来自Vataireopsis araroba (Aguiar) Ducke 的 GalNAc/Gal 特异性凝集素的纯化和表征，指定为 VaL。使用瓜耳胶亲和色谱一步从种子中纯化凝集素。凝集素在 SDS-PAGE 上以约 35 kDa 的单个条带迁移，并且在自然条件下以同型二聚体形式出现。纯化的凝集素在高达 60 °C 的温度和 7 至 8 的 pH 范围内稳定，需要二价阳离子才能发挥其活性。糖抑制试验证明了凝集素对N- 的特异性乙酰-D-半乳糖胺、D-半乳糖和相关糖类。此外，聚糖阵列分析显示 VaL 优先与含有末端 GalNAc/Galβ1-4GlcNAc 的聚糖相互作用。使用三种昆虫细胞系进行生物活性测定：来自云杉芽虫Choristoneura fumiferana的 CF1 中肠细胞、来自果蝇Drosophila melanogaster的 S2 胚胎细胞和来自玉米穗虫Helicoverpa zea的 GutAW 中肠细胞. 体外试验表明 VaL 对 CF1 细胞有生物抑制作用，但对 S2 和 GutAW 细胞没有影响。凝集素通过减少细胞生长和诱导细胞凝集来呈现生物静态效应，表明与细胞表面上的聚糖相互作用。VaL 已被表征为黄檀科部落的半乳糖苷特异性凝集素，与来自Vatairea和Arachis的凝集素具有序列相似性。