BCL2-like 10 (BCL2L10) is abundantly expressed in mammalian oocytes and plays a crucial role in the completion of oocyte meiosis. However, the expression patterns of BCL2L10 and its biological functions during preimplantation development have not been well characterized. Here, we investigated the spatiotemporal expressions of Bcl2l10 during mouse preimplantation development using RT-qPCR and immunofluorescence and its biological function using siRNA and morpholino injection into pronuclear embryos. Results from RT-qPCR showed that Bcl2l10 was highly expressed in the metaphase Ⅱ-stage oocytes and pronuclear-stage embryos, but expression markedly decreased from the two-cell stage onwards and was no longer detected at the four-cell stage and beyond. Immunofluorescence staining showed that BCL2L10 was detectable throughout preimplantation development and localized in the cytoplasm and nuclei. Knocking down Bcl2l10 resulted in a reduced blastocyst formation rate (P < 0.01) and decreased expression of OCT4, NANOG, and SOX17 (P < 0.05). We concluded that the role of BCL2L10 is strongly associated with developmental competence of preimplantation mouse embryos.

BCL2-like 10 (BCL2L10) 在哺乳动物卵母细胞中大量表达，在卵母细胞减数分裂的完成中起着至关重要的作用。然而，BCL2L10 的表达模式及其在植入前发育过程中的生物学功能尚未得到很好的表征。在这里，我们使用 RT-qPCR 和免疫荧光研究了Bcl2l10在小鼠植入前发育过程中的时空表达，以及使用 siRNA 和吗啉代注射到原核胚胎中的生物学功能。RT-qPCR 的结果显示Bcl2l10在中期Ⅱ期卵母细胞和原核期胚胎中高表达，但从二细胞期开始表达明显下降，四细胞期及以后不再检测到。免疫荧光染色显示 BCL2L10 在整个植入前发育过程中都可检测到，并定位于细胞质和细胞核中。敲低Bcl2l10导致囊胚形成率降低 ( P  < 0.01) 并降低 OCT4、NANOG 和 SOX17 的表达 ( P  < 0.05)。我们得出结论，BCL2L10 的作用与植入前小鼠胚胎的发育能力密切相关。