Cancer initiation and progression are profoundly along with the crosstalk between cancer cells and the surrounding stroma. Accumulating evidence has shown that the therapy targeting the extracellular matrix (ECM) would regress tumor growth and invasion in the most common carcinomas. However, it remains largely unexplored in several rare tumors like odontogenic tumors. Ameloblastoma (AM) is the representative odontogenic epithelial tumor in the jawbone, and it usually infiltrates into adjacent bone marrow and has unlimited growth capacity and a high potential for recurrence. This study aims to investigate the role of collagen-rich ECM during the invasion of AM. Transcriptomic analysis revealed that ECM- and epithelial-to-mesenchymal transition (EMT)-related genes were up-regulated in AM compared to ameloblastoma cell line, AM-1. Tumoroid forming analysis showed that Collagen-rich ECM is indispensable for AM progression, especially for aggressive growth patterns and collective invasion.

中文翻译：

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成釉细胞瘤和 AM-1 细胞系的转录组学比较分析。

癌症的发生和发展与癌细胞和周围基质之间的相互作用密切相关。越来越多的证据表明，靶向细胞外基质 (ECM) 的疗法可以抑制最常见癌症的肿瘤生长和侵袭。然而，在牙源性肿瘤等几种罕见肿瘤中，它在很大程度上仍未得到探索。成釉细胞瘤（ameloblastoma，AM）是颌骨中具有代表性的牙源性上皮性肿瘤，通常浸润到邻近的骨髓中，具有无限的生长能力和较高的复发可能性。本研究旨在探讨富含胶原蛋白的 ECM 在 AM 侵袭过程中的作用。转录组学分析显示，与成釉细胞瘤细胞系 AM-1 相比，ECM 和上皮间质转化 (EMT) 相关基因在 AM 中上调。