Although glyphosate (Gly) is one of the most widely used agrochemicals, it is also of the most difficult to measure. Gly, its metabolites, and related compounds cannot be sought within the scope of multi-residue methods. Specific so-called single-residue methods are used instead. Liquid chromatography-mass spectrometry (LC-MS) is currently the most widely used technique for determining Gly and its metabolites. This review addresses the different LC-MS-based methods proposed for the determination of Gly and related species in food and environment matrices. Sample preparation (food and environment), as well as their determination based in novel liquid chromatography/mass spectrometry approaches including different specific stationary phases are presented and the specific analytical challenges, strengths and drawbacks are critically discussed.

尽管草甘膦 (Gly) 是使用最广泛的农用化学品之一，但它也是最难测量的。Gly、其代谢物和相关化合物不能在多残留方法的范围内寻找。取而代之的是使用特定的所谓单残基方法。液相色谱-质谱法 (LC-MS) 是目前应用最广泛的 Gly 及其代谢物测定技术。本综述介绍了为测定食品和环境基质中的甘氨酸和相关物质而提出的不同的基于 LC-MS 的方法。介绍了样品制备（食品和环境）及其基于新型液相色谱/质谱法（包括不同特定固定相）的测定，并批判性地讨论了具体的分析挑战、优点和缺点。