Cell-based Surface Plasmon Resonance imaging (SPRi) with anti-endoglin VHH antibodies microarray on a gold-covered biochip was used to characterize the binding to endoglin expressed on the surface membrane of SC cells. SPRi showed the specific capture of SC cells by the immobilized anti-endoglin VHHs, but not of THP-1 cells that do not express endoglin on their plasmatic membrane. The SPRi reflectivity shifts with SC cells bound by anti-endoglin VHHs ranged from 3.59 to 5.54% (average 4.66 ± 1.18%) and with THP-1 cells from 2.23 to 3.05% (average 2.62 ± 1.32% (p < 0.001)) and the SC cell densities with anti-endoglin VHHs ranged from 417 to 747.7, (average 548 cells/2.28 × 10⁵ μm² ± 132). Anti-endoglin VHHs bind epitopes located in the extracellular domains of endoglin with different avidity as suggested by the differences in SC cell densities. The cell-based SPRi of anti-endoglin VHH microarrays can be used both in studies aimed at a better understanding of endoglin function in living cells and in VHH applications as nanoprobes.

基于细胞的表面等离子共振成像 (SPRi) 与金覆盖的生物芯片上的抗内皮糖蛋白 VHH 抗体微阵列用于表征与 SC 细胞表面膜上表达的内皮糖蛋白的结合。SPRi 显示固定化抗内皮糖蛋白 VHH 对 SC 细胞的特异性捕获，而不是不在其质膜上表达内皮糖蛋白的 THP-1 细胞的特异性捕获。SPRi 反射率随抗内皮糖蛋白 VHH 结合的 SC 细胞变化范围为 3.59 至 5.54%（平均 4.66 ± 1.18%），THP-1 细胞变化范围为 2.23 至 3.05%（平均 2.62 ± 1.32%（p  < 0.001））和具有抗内皮糖蛋白 VHH 的 SC 细胞密度范围为 417 至 747.7，（平均 548 个细胞/2.28 × 10 ⁵  μm ² ± 132). SC 细胞密度的差异表明，抗内皮糖蛋白 VHH 以不同的亲和力结合位于内皮糖蛋白胞外域的表位。抗内皮糖蛋白 VHH 微阵列的基于细胞的 SPRi 可用于旨在更好地了解活细胞内皮糖蛋白功能的研究以及作为纳米探针的 VHH 应用。