Surface plasmon resonance (SPR) has been utilized extensively in bioanalysis since the early 90s due to its unique combination of label-free, highly sensitive, and robust detection. In recent years, SPR has been coupled with and complimented by several other techniques that provide the qualitative and molecular information that SPR lacks. In this paper, advances in studies that present molecular identification to quantitative measurements by SPR are reviewed. In particular, coupling of SPR measurements and MALDI mass spectrometry (MALDI-MS) has been broadly explored where plasmon-assisted ionization upon gold substrates appears to demonstrate many added benefits. Imaging SPR analysis, upon coupling, has contributed to new analytical capability that proves to be powerful with elevated throughput. The combination of SPR and Raman spectroscopy has also found a wide range of applications in providing molecular information that promotes quantitative analysis. Similar to MALDI, the plasmonic thin-film metal substrates provide a desired surface structure and property for Raman enhancement, improving subsequent molecular recognition. Instrumentation advancements and improvements in coupling efficiency has facilitated a series of SPR sensor developments where the plasmonic properties of the surface contribute uniquely to the performance of the coupled technique.

自 90 年代初以来，表面等离子共振 (SPR) 技术因其无标记、高灵敏度和稳健检测的独特组合而被广泛用于生物分析。近年来，SPR 与其他几种技术相结合并得到了补充，这些技术提供了 SPR 所缺乏的定性和分子信息。在本文中，综述了将分子鉴定呈现给 SPR 定量测量的研究进展。特别是，SPR 测量和 MALDI 质谱 (MALDI-MS) 的耦合已得到广泛探索，其中金基板上的等离子体辅助电离似乎证明了许多额外的好处。耦合后的成像 SPR 分析促成了新的分析能力，该能力被证明在提高吞吐量的情况下非常强大。SPR 和拉曼光谱的结合在提供促进定量分析的分子信息方面也有广泛的应用。与 MALDI 类似，等离子体薄膜金属基板为拉曼增强提供了所需的表面结构和特性，从而改善了后续的分子识别。仪器的进步和耦合效率的改进促进了一系列 SPR 传感器的开发，其中表面的等离子体特性对耦合技术的性能有独特的贡献。