Multiple lesions in the same vessel is one of the most common situations found in patients suffering from cardiovascular diseases, this complicates not only the assessment of the severity of each one but also their treatment. To date, the effect of multiple stenoses on different parameters has been simulated by numerical studies. Few others have implemented in vitro platforms for their investigation. However, visualization of thrombosis formation in this kind of lesion is still needed. This in vitro study monitors the formation of thrombus inside microchannels having one, two, and three stenoses. Whole blood was perfused through each channel at high shear rates (> 12,000 s−1), generating thrombosis. Flow changes across each lesion as well as the final percentage of aggregations were monitored. Thus, the location where total occlusion could be produced was found to be the first stenosis for all the cases. Less flow reaching the second and third stenoses was observed which demonstrates that aggregations were growing at the first one. This was verified by measuring the percentage of aggregations at the end of the test.

中文翻译：

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多处狭窄对血栓形成的影响：一项体外研究

同一血管中的多个病变是心血管疾病患者中最常见的情况之一，这不仅使评估每一个病变的严重程度变得复杂，而且使它们的治疗变得复杂。迄今为止，已经通过数值研究模拟了多处狭窄对不同参数的影响。很少有人为他们的调查实施了体外平台。然而，仍然需要对这种病变中的血栓形成进行可视化。这项体外研究监测了具有一个、两个和三个狭窄的微通道内血栓的形成。全血以高剪切率 (> 12,000 s-1) 通过每个通道灌注，产生血栓形成。监测每个病变的流量变化以及聚集的最终百分比。因此，发现可能产生完全闭塞的位置是所有病例的第一个狭窄。观察到到达第二个和第三个狭窄处的流量较少，这表明聚集在第一个狭窄处生长。这通过在测试结束时测量聚集的百分比来验证。