Jumbo bacteriophages, which have genomic DNA larger than 200 k-bp, are attracting attention as potential biocontrol agents to suppress bacterial diseases in cultivated crops because they generally have a broader host range of phytopathogenic bacteria compared with ordinary bacteriophages. Thus, the identification of new jumbo phages from environmental and agricultural resources is required to develop effective biological control materials. In a survey of phages targeting the phytopathogenic bacterium Burkholderia glumae, bacteriophages FLC8, FLC9, and FLC10 were isolated from fallen leaf compost. All three phages had an icosahedral head and tail structure. FLC8 and FLC9 had 225,545-bp and 321,833-bp genomic DNA, respectively, indicating that they could be classified as jumbo phages, whereas FLC10 had 32,867-bp genomic DNA, indicating that it was an ordinary phage. All three phages clearly suppressed rice seedling rot disease caused by B. glumae: the control values were 77.1 of FLC8, 82.1 of FLC9 and 33.3 of FLC10, respectively. FLC8 and FLC9 could infect five B. glumae and six B. plantarii strains, which were all strains using analysis of phage host range, while FLC10 had a narrow host range. Evaluation of the preservation condition of both jumbo phages revealed that ~ 90% infectivity of them was maintained in phage buffer (pH 7.5) at 4 °C for ~ 30 days.

基因组 DNA 大于 200 k-bp 的巨型噬菌体作为抑制栽培作物细菌性病害的潜在生物防治剂而受到关注，因为与普通噬菌体相比，它们通常具有更广泛的植物病原细菌宿主范围。因此，需要从环境和农业资源中鉴定新的巨型噬菌体以开发有效的生物控制材料。在针对植物病原细菌Burkholderia glumae的噬菌体调查中, 从落叶堆肥中分离出噬菌体 FLC8、FLC9 和 FLC10。所有三种噬菌体都具有二十面体的头尾结构。FLC8和FLC9分别有225,545-bp和321,833-bp的基因组DNA，表明它们可以归类为巨型噬菌体，而FLC10有32,867-bp的基因组DNA，表明它是普通噬菌体。所有三种噬菌体都明显抑制了由谷粒芽孢杆菌引起的水稻秧苗腐烂病：对照值分别为 FLC8 的 77.1、FLC9 的 82.1 和 FLC10 的 33.3。FLC8 和 FLC9 可感染 5 种谷壳芽孢杆菌和 6种植物芽孢杆菌菌株，它们都是使用噬菌体宿主范围分析的菌株，而 FLC10 的宿主范围较窄。对两种巨型噬菌体保存条件的评估表明，它们的 ~ 90% 感染性在 4 °C 的噬菌体缓冲液 (pH 7.5) 中保持了 ~ 30 天。