Vibrio alginolyticus has been described as a pathogenic species that can cause vibriosis in shrimp and many marine fishes. A total of nine V. alginolyticus isolates were segregated from 25 water samples collected from various locations at Muttukadu brackishwater lagoon in Tamil Nadu, India. The isolates were separated and characterised phenotypically and genotypically and tested for antibiotic resistance against a range of 14 antimicrobial agents. All the isolates were resistant to the penicillin group of antibiotics. The isolates exhibited higher rates of resistance against antibiotics such as chloramphenicol, nalidixic acid, cefuroxime, cefoperazone, and cefazolin and moderately resistant against tetracycline, tobramycin, gentamicin, cefepime, and showed lower degree resistance against doxycycline, ciprofloxacin and gatifloxacin. The MAR index of the isolates (0.285 to 0.5) indicated that they have originated from high-risk sources of contamination. Genome fingerprinting with RAPD and ERIC-PCR showed that RAPD was highly discriminatory than ERIC-PCR for the tested isolates. Further, both fingerprinting analyses revealed a high level of genetic diversity among the isolates. These data unequivocally indicated that the population of V. alginolyticus in a brackishwater environment was not dominated by any environmentally adapted strains and there was mixing and dispersal of genotypes within water samples.

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Muttukadu 咸水泻湖溶藻弧菌的表型特征和菌株分化

溶藻弧菌已被描述为一种致病菌种，可引起虾和许多海鱼的弧菌病。从印度泰米尔纳德邦 Muttukadu 咸水泻湖的不同地点收集的 25 个水样中分离出总共 9 个溶藻弧菌分离株。对分离株进行了表型和基因型分离和表征，并测试了对一系列 14 种抗菌药物的抗生素耐药性。所有分离株均对青霉素类抗生素具有耐药性。分离株对氯霉素、萘啶酸、头孢呋辛、头孢哌酮、头孢唑林等抗生素耐药率较高，对四环素、妥布霉素、庆大霉素、头孢吡肟等耐药率较高，对强力霉素、环丙沙星和加替沙星耐药率较低。分离株的 MAR 指数（0.285 至 0.5）表明它们来自高风险污染源。使用 RAPD 和 ERIC-PCR 进行基因组指纹分析表明，对于测试的分离株，RAPD 比 ERIC-PCR 具有更高的辨别力。此外，两种指纹分析都揭示了分离株之间的高水平遗传多样性。这些数据明确表明，微咸水环境中的溶藻弧菌种群不受任何适应环境的菌株支配，并且水样中的基因型存在混合和分散。两种指纹分析都揭示了分离株之间的高水平遗传多样性。这些数据明确表明，微咸水环境中的溶藻弧菌种群不受任何适应环境的菌株支配，并且水样中的基因型存在混合和分散。两种指纹分析都揭示了分离株之间的高水平遗传多样性。这些数据明确表明，微咸水环境中的溶藻弧菌种群不受任何适应环境的菌株支配，并且水样中的基因型存在混合和分散。