Nafion®-nanostructured polyaniline (nsPANi) composite film is prepared using cyclic voltammetry (CV) and immobilized with creatinine deiminase (CD) enzyme and is used to sense creatinine in a buffer phosphate solution. The conditions for preparing Nafion®-nsPANi composite film are optimized by using a mixture design for which the sensitivity is the response. The relationship between the sensitivity of the amperometric creatinine biosensor (y) and the normalized aniline concentration (Y₁), HCl concentration (Y₂) and scanning rate (Y₃) is y = 119.44Y₁ + 45.23Y₂ + 100.93Y₃ + 255.69Y₁Y₂ + 313.16Y₁Y₃ + 430.56Y₁Y₂Y₃

The maximum sensitivity of an amperometric creatinine biosensor that is constructed using Nafion®-nsPANi composite film in 0.0943 M aniline, 0.9024 M HCl and using a scanning rate of 27.88 mV s ^− 1 is 2013.2 μA mM⁻¹ cm⁻², which is 54.9% better than the sensitivity of a conventional experimental technique. The amperometric creatinine biosensor is 6.60% less sensitive after sensing 0.15 mM creatinine 240 times. The amperometric creatinine biosensor incurs insignificant interference in 0.138 mM urea, 0.085 mM ascorbic acid (AA) and 5.54 mM glucose.

Nafion®-纳米结构聚苯胺 (nsPANi) 复合膜是使用循环伏安法 (CV) 制备的，并固定有肌酐脱亚胺酶 (CD) 酶，用于检测磷酸盐缓冲溶液中的肌酐。Nafion®-nsPANi 复合膜的制备条件通过使用灵敏度为响应的混合物设计进行了优化。安培法肌酐生物传感器的灵敏度（y）与归一化苯胺浓度（Y ₁）、HCl浓度（Y ₂）和扫描速率（Y ₃）的关系为y  = 119.44 Y ₁  + 45.23 Y ₂  + 100.93 Y₃  + 255.69 Y ₁ Y ₂  + 313.16 Y ₁ Y ₃  + 430.56 Y ₁ Y ₂ Y ₃

使用 Nafion®-nsPANi 复合膜在 0.0943 M 苯胺、0.9024 M HCl 中并使用 27.88 mV s  ^{−  1}的扫描速率构建的安培肌酸酐生物传感器的最大灵敏度为 2013.2 μA mM ⁻¹ cm ⁻²，即 54.9 % 优于传统实验技术的灵敏度。在检测 0.15 mM 肌酐 240 次后，安培法肌酐生物传感器的灵敏度降低了 6.60%。安培法肌酐生物传感器对 0.138 mM 尿素、0.085 mM 抗坏血酸 (AA) 和 5.54 mM 葡萄糖的干扰很小。