Marcia hiantina (Mollusca, Bivalvia) (Lamarck, 1818), is an edible clam mainly distributed along the tropical coastal regions. Recent researches have demonstrated that clams can possess compounds, including polysaccharides, with a wide range of biological actions including antioxidant, immunomodulatory and antitumor activities. Here an α-glucan was isolated from M. hiantina by hot water, purified by anion exchange chromatography, and its structure was characterized by a combination of multiple nuclear magnetic resonance (NMR) methods (1D ¹H, ¹H-¹H COSY, ¹H-¹H TOCSY, ¹H-¹H NOESY, ¹H-¹³C HSQC and ¹H-¹³C HSQC-NOESY spectra), gas chromatography-mass spectrometry, and high performance size exclusion chromatography (HPSEC). The analysis from NMR, monosaccharide composition, methylation analyses and HPSEC combined with multi-angle light scattering (MALS) of M. hiantina-derived α-glycan confirmed a branched polysaccharide exclusively composed of glucose (Glc), mostly 4-linked in its backbone, branched occasionally at 6-positions, and having a molecular weight of ~ 570 kDa. The mollusk α-glucan was subjected to four cell-based assays: (i) viability of three cell lines (RAW264.7, HaCaT, and HT-29), (ii) activity on lipopolysaccharide (LPS)-induced prostaglandin production in RAW264.7 cells, (iii) inhibitory activities of in H₂O₂- and LPS-induced reactive oxygen species (ROS) production in HMC3 cells, and (iv) HaCaT cell proliferation. Results have indicated no cytotoxicity, potent inhibition of both H₂O₂- and LPS-induced ROS, and potent cell proliferative activity.

Marcia hiantina (Mollusca, Bivalvia) (Lamarck, 1818)，是一种主要分布于热带沿海地区的食用蛤。最近的研究表明，蛤蜊可以含有多种化合物，包括多糖，具有广泛的生物作用，包括抗氧化、免疫调节和抗肿瘤活性。这里通过热水从M. hiantina中分离出 α-葡聚糖，通过阴离子交换色谱纯化，并通过多种核磁共振 (NMR) 方法（1D ¹ H、¹ H- ¹ H COSY、¹ H- ¹ H TOCSY，¹ H- ¹ H NOESY，¹ H-¹³ C HSQC 和¹ H- ¹³ C HSQC-NOESY 光谱）、气相色谱-质谱法和高效体积排阻色谱法 (HPSEC)。来自M. hiantina的α-聚糖的核磁共振、单糖组成、甲基化分析和 HPSEC 结合多角度光散射 (MALS) 的分析证实了一种完全由葡萄糖 (Glc) 组成的支链多糖，其主链中大部分是 4-连接的，偶尔在 6 位分枝，分子量约为 570 kDa。对软体动物 α-葡聚糖进行四种基于细胞的测定：(i) 三种细胞系（RAW264.7、HaCaT 和 HT-29）的活力，(ii) RAW264 中脂多糖 (LPS) 诱导的前列腺素产生的活性.7 个细胞，(iii) 在 H 中的抑制活性_{HMC3 细胞中2} O ₂ - 和 LPS 诱导的活性氧 (ROS) 产生，以及 (iv) HaCaT 细胞增殖。结果表明没有细胞毒性，有效抑制 H ₂ O ₂ - 和 LPS 诱导的 ROS，以及有效的细胞增殖活性。