We reported the efficacy of the Iron nanoparticles (IONPs) and assessed two different approaches for the synthesis of IONPs i.e. Polyol and co-precipitation method and further, evaluate their antimicrobial properties. Ferrous sulphate heptahydrate salts were reduced with ethylene glycol to obtain IONP and Fe+2 and Fe+3 iron precursor salts were used for co-precipitation reaction that was performed with KOH at optimum heating. Further, synthesized (IONPs) were characterized by hydrodynamic radii measurement done by DLS clearly indicating the size of IONPs is 79.75 nm in polyol based and 135.1 nm in co-precipitation method. The biological efficacy in terms of antimicrobial activity was assessed by the Kirby Bauer method, applied for both Gram-positive and Gram-negative bacteria such as Staphylococcus aureus and Escherichia coli, respectively. The ZOI values i.e. Zone of inhibition diameter was found to be clearly visible in both S. aureus and E. coli, indicating bactericidal activity. Further growth kinetics studies and bacterial genotoxicity was also assessed. Hence, IONPs synthesized are proposed to have great potential as an antibacterial agent and can be used in drug delivery.

中文翻译：

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纳米铁的合成、表征及抗菌活性评价

我们报告了铁纳米粒子 (IONPs) 的功效，并评估了两种不同的 IONPs 合成方法，即多元醇和共沉淀法，并进一步评估了它们的抗菌性能。用乙二醇还原七水硫酸亚铁盐以获得 IONP，并将 Fe+2 和 Fe+3 铁前体盐用于与 KOH 在最佳加热条件下进行的共沉淀反应。此外，合成的 (IONPs) 通过 DLS 进行的流体动力学半径测量来表征，清楚地表明 IONPs 的尺寸在多元醇基中为 79.75 nm，在共沉淀法中为 135.1 nm。通过 Kirby Bauer 方法评估抗菌活性方面的生物学功效，分别适用于革兰氏阳性菌和革兰氏阴性菌，如金黄色葡萄球菌和大肠杆菌。ZOI 值，即抑菌圈直径被发现在金黄色葡萄球菌和大肠杆菌中清晰可见，表明具有杀菌活性。还评估了进一步的生长动力学研究和细菌遗传毒性。因此，合成的 IONPs 被认为具有作为抗菌剂的巨大潜力，可用于药物输送。