The development of simple sensing platforms for evaluating the oral conditions has attracted great attentions for healthcare. Saliva or toothpaste contains various analytes that can indicate health conditions. A salivary glucose sensing platform can provide a convenient and non-invasive alternative detection approach for diabetic patients. Toothbrush is used every day, and it has an easy access to saliva biomarker and toothpaste residues. Here, we demonstrate for the first time an amperometric biosensor on a toothbrush, using glucose as a typical analyte. The carbon graphite ink and the Ag/AgCl ink are painted on a toothbrush as the sensing electrodes, followed by the enzyme immobilization. The sensor shows an excellent detection performance for glucose with a concentration ranging from 0.18 mM to 5.22 mM and a short detection time of less than 5 min. The sensor is promising for the non-invasive monitoring of salivary glucose levels in diabetic patients when they brush their teeth. We anticipate that these results would open up exciting opportunities for developing new toothbrush sensors, as well as advance related healthcare applications.

用于评估口腔状况的简单传感平台的开发引起了医疗保健领域的极大关注。唾液或牙膏中含有各种可以指示健康状况的分析物。唾液葡萄糖传感平台可以为糖尿病患者提供一种方便且无创的替代检测方法。牙刷每天都在使用，很容易接触到唾液生物标志物和牙膏残留物。在这里，我们首次展示了牙刷上的安培生物传感器，使用葡萄糖作为典型分析物。将碳石墨墨水和Ag/AgCl墨水涂在牙刷上作为传感电极，然后进行酶固定化。该传感器对浓度范围为 0.18 mM 至 5 mM 的葡萄糖表现出优异的检测性能。22 mM 和小于 5 分钟的短检测时间。该传感器有望在糖尿病患者刷牙时无创监测唾液葡萄糖水平。我们预计这些结果将为开发新的牙刷传感器以及推进相关医疗保健应用开辟令人兴奋的机会。