Introduction: Apelin could be one of the last protective defenses before developing obesity-related disorders, including insulin resistance, type 2 diabetes, and hypertension, which can be modified by dietary intake. The present study investigated the association of habitual intake of total fatty acids (TFAs), saturated-, monounsaturated-, polyunsaturated FAs, n-3, and n-6 FAs with Apelin expression in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT). Methods: We obtained VAT and SAT from 168 participants (64 nonobese and 104 obese) who had undergone open abdominal surgery. Dietary intake information was gathered with a valid and reliable food frequency questionnaire. The mRNA expression of the Apelin gene was analyzed by real-time PCR. Results: Apelin serum levels were increased in the obese subjects compared to the nonobese group (p = 0.016). The SAT and VAT Apelin mRNA levels were significantly elevated in the obese participants compared to the nonobese ones (p #x3c; 0.05). Based on BMI status, only obese subjects indicated a positive association between SAT and VAT Apelin expression and TFA intake (p #x3c; 0.001). However, this association was observed between SAT and VAT Apelin gene expression and polyunsaturated fatty acid (PUFA) and n-3 FA intakes in both obese and nonobese groups (p #x3c; 0.05). Conclusion: High Apelin gene expression was associated with TFA intake in obese subjects in both fat tissues. However, habitual intake of PUFA and n-3 FA was associated with Apelin gene expression in obese and nonobese individuals. Our results indicate a determinative role of the quality and quantity of FA intake on adipose tissue.
Public Health Genomics

中文翻译：

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脂肪的习惯性饮食摄入与肥胖成人内脏和皮下脂肪组织中的 Apelin 基因表达及其血清水平有关吗？

简介： Apelin 可能是发生肥胖相关疾病（包括胰岛素抵抗、2 型糖尿病和高血压）之前最后的保护性防御措施之一，这些疾病可以通过饮食摄入来改变。本研究调查了习惯性摄入总脂肪酸 (TFA)、饱和脂肪酸、单不饱和脂肪酸、多不饱和脂肪酸、n-3 和 n-6 脂肪酸与内脏脂肪组织 (VAT) 和皮下脂肪组织中 Apelin 表达的关联。坐）。方法：我们从接受过开腹手术的 168 名参与者（64 名非肥胖者和 104 名肥胖者）中获得了 VAT 和 SAT。使用有效且可靠的食物频率问卷收集膳食摄入量信息。通过实时PCR分析Apelin基因的mRNA表达。结果：与非肥胖组相比，肥胖受试者的 Apelin 血清水平升高 ( p = 0.016)。与非肥胖参与者相比，肥胖参与者的 SAT 和 VAT Apelin mRNA 水平显着升高 ( p #x3c; 0.05)。根据 BMI 状态，只有肥胖受试者表明 SAT 和 VAT Apelin 表达与 TFA 摄入量呈正相关（p #x3c；0.001）。然而，在肥胖和非肥胖组中，在 SAT 和 VAT Apelin 基因表达与多不饱和脂肪酸 (PUFA) 和 n-3 FA 摄入量之间观察到了这种关联 (p #x3c; 0.05 )。结论：高 Apelin 基因表达与肥胖受试者两种脂肪组织中的 TFA 摄入量相关。然而，习惯性摄入 PUFA 和 n-3 FA 与肥胖和非肥胖个体的 Apelin 基因表达相关。我们的结果表明 FA 摄入量的质量和数量对脂肪组织具有决定性作用。
公共卫生基因组学