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Monitoring the Spread of Potato Virus Diseases in Kazakhstan
American Journal of Potato Research ( IF 1.5 ) Pub Date : 2022-12-08 , DOI: 10.1007/s12230-022-09895-y
Dias Daurov , Assel Argynbayeva , Ainash Daurova , Kuanysh Zhapar , Zagipa Sapakhova , Kabyl Zhambakin , Malika Shamekova

Potato (Solanum tuberosum L.) is one of the main food crops worldwide, including in Kazakhstan. Viral diseases are a major constraint to sustainable potato production, as they cause large losses in crop quantity and quality. To determine the current status of potato production in Kazakhstan, we studied tuber and leaf samples in large seed and commercial farms of the Republic. Samples of tuber and leaf samples randomly sampled were tested for five main viruses: potato virus Y (PVY), potato virus X (PVX), potato virus M (PVM), potato virus S (PVS), and potato leafroll virus (PLRV). A multiplex reverse transcription polymerase chain reaction (mpRT-PCR) was used to monitor viral infection. During the monitoring of tuber samples, it was found that the most common virus in almost all regions was the PVS virus at 64.5%. Moreover, most of the viruses originated in commercial farms. As a result of the analysis of leaves selected in the fields, it was found that the most common viruses in the regions were PVM at 46% and PVS at 35.3%. The work performed to monitor viral diseases in various regions of Kazakhstan will enable direct actions to maintain and improve the phytosanitary status of potatoes in Kazakhstan in the future.



中文翻译:

监测马铃薯病毒病在哈萨克斯坦的传播

马铃薯 ( Solanum tuberosumL.) 是世界范围内的主要粮食作物之一,包括在哈萨克斯坦。病毒病是马铃薯可持续生产的主要制约因素,因为它们会造成作物数量和质量的巨大损失。为了确定哈萨克斯坦马铃薯生产的现状,我们研究了共和国大型种子和商业农场的块茎和叶样本。对随机抽取的块茎和叶样品进行五种主要病毒检测:马铃薯病毒 Y (PVY)、马铃薯病毒 X (PVX)、马铃薯病毒 M (PVM)、马铃薯病毒 S (PVS) 和马铃薯卷叶病毒 (PLRV) . 多重逆转录聚合酶链反应 (mpRT-PCR) 用于监测病毒感染。在对块茎样本的监测中,发现几乎所有地区最常见的病毒是PVS病毒,占64.5%。而且,大多数病毒起源于商业农场。通过对田间选择的叶子进行分析,发现该地区最常见的病毒是 PVM,占 46%,PVS 占 35.3%。在哈萨克斯坦各个地区开展的监测病毒病的工作将使哈萨克斯坦未来采取直接行动来维持和改善马铃薯的植物检疫状况。

更新日期:2022-12-09
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