Our previous studies showed that Pumilio RNA-binding family member 1 (PUM1) gene is abnormally expressed in pancreatic cancer (PC) tissues, and its knockdown suppresses the growth and metastasis of PC cells. Here, we aimed to further investigate its role in angiogenesis. Immunohistochemical assays were carried out to analyze CD31 and PUM1 expression levels in PC tissues and in subcutaneous xenograft tumors. CD31 levels in PC tissues are expressed as microvessel density (MVD). MVD value was positively correlated with PUM1 protein expression. PUM1 was successfully overexpressed or silenced in the PC cell lines. The proliferation, migration, invasion, and tube formation ability of HUVECs were enhanced when cocultured with PC cells overexpressing PUM1. PUM1 overexpression increased extracellular and intracellular VEGFA protein levels in PC cells. Moreover, angiogenesis-related signaling in HUVECs was activated when HUVECs were cocultured with PC cells overexpressing PUM1. Nevertheless, PC cells silenced with PUM1 had the opposite effect. Moreover, subcutaneous xenograft tumors overexpressing PUM1 have the higher expression level of CD31, while subcutaneous xenograft tumors silencing PUM1 have the lower expression level of CD31. In conclusion, PUM1 in PC cells may play a promoting role in PC angiogenesis. PUM1 may be a new regulator of angiogenesis in PC cells.

中文翻译：

---

Pumilio RNA 结合家族成员 1 对胰腺癌血管生成的促进作用

我们之前的研究表明，Pumilio RNA 结合家族成员 1 (PUM1) 基因在胰腺癌 (PC) 组织中异常表达，其敲低抑制了 PC 细胞的生长和转移。在这里，我们旨在进一步研究其在血管生成中的作用。进行免疫组织化学测定以分析 PC 组织和皮下异种移植肿瘤中的 CD31 和 PUM1 表达水平。PC 组织中的 CD31 水平表示为微血管密度 (MVD)。MVD值与PUM1蛋白表达呈正相关。PUM1 在 PC 细胞系中成功过表达或沉默。当与过表达 PUM1 的 PC 细胞共培养时，HUVEC 的增殖、迁移、侵袭和管形成能力得到增强。PUM1 过表达增加了 PC 细胞中的细胞外和细胞内 VEGFA 蛋白水平。此外，当 HUVEC 与过表达 PUM1 的 PC 细胞共培养时，HUVEC 中的血管生成相关信号被激活。然而，用 PUM1 沉默的 PC 细胞具有相反的效果。此外，过表达PUM1的皮下异种移植肿瘤具有较高的CD31表达水平，而沉默PUM1的皮下异种移植肿瘤具有较低的CD31表达水平。总之，PC细胞中的PUM1可能在PC血管生成中起到促进作用。PUM1 可能是 PC 细胞中血管生成的新调节剂。而皮下异种移植瘤中沉默PUM1的CD31表达水平较低。总之，PC细胞中的PUM1可能在PC血管生成中起到促进作用。PUM1 可能是 PC 细胞中血管生成的新调节剂。而皮下异种移植瘤中沉默PUM1的CD31表达水平较低。总之，PC细胞中的PUM1可能在PC血管生成中起到促进作用。PUM1 可能是 PC 细胞中血管生成的新调节剂。