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Weizmannia acidilactici sp. nov., a lactic acid producing bacterium isolated from soils
Systematic and Applied Microbiology ( IF 3.4 ) Pub Date : 2022-12-14 , DOI: 10.1016/j.syapm.2022.126389
Vasana Tolieng 1 , Naoto Tanaka 2 , Yuh Shiwa 2 , Sitanan Thitiprasert 1 , Pawina Kanchanasin 3 , Wongsakorn Phongsopitanun 3 , Auttaporn Booncharoen 4 , Nuttha Thongchul 1 , Somboon Tanasupawat 3
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The strains designed PP-18T, JC-4 and JC-7 isolated from soils, were Gram-stain-positive rods, facultative anaerobe, endospore-forming bacteria. The strains produced l-lactic acid from glucose. They showed positive for catalase but negative for oxidase, nitrate reduction and arginine hydrolysis. Strains P-18T, JC-4 and JC-7 were closely related to Weizmannia coagulans LMG 6326T (97.27–97.64%) and W. acidiproducens KCTC 13078T (96.46–96.74%) based on 16S rRNA gene sequence similarity, respectively. They contained meso-diaminopimelic acid in cell wall peptidoglycan and had seven isoprene units (MK-7) as the predominant menaquinone. The major cellular fatty acids of strain PP-18T were iso-C15:0, anteiso-C17:0, iso-C16:0 and anteiso-C15:0. The ANIb and ANIm values among the genomes of strains PP-18T, JC-4 and JC-7 are above 99.4% while their ANIb and ANIm values among them and W. coagulans LMG 6326T and W. acidiproducens KCTC 13078T were ranged from 76.61 to 79.59%. These 3 strains showed the digital DNA-DNA hybridization (dDDH) values of 20.7–23.6% when compared with W. coagulans LMG 6326T and W. acidiproducens DSM 23148T. The DNA G + C contents of strains PP-18T, JC-4 and JC-7 were 45.82%, 45.86% and 45.86%, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphoglycolipids. The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis indicated that the strains PP-18T, JC-4 and JC-7 should be represented as a novel species within the genus Weizmannia for which the name Weizmannia acidilactici sp. nov. is proposed. The type strain is PP-18T (=KCTC 33974T = NBRC 113028T = TISTR 2515T).



中文翻译:

Weizmannia acidilactici sp。nov., 一种从土壤中分离出来的产乳酸菌

从土壤中分离得到的设计菌株PP- 18T、JC-4和JC-7均为革兰氏染色阳性杆菌、兼性厌氧菌、内生孢子菌。这些菌株从葡萄糖中产生l-乳酸。它们对过氧化氢酶呈阳性,但对氧化酶、硝酸盐还原和精氨酸水解呈阴性。基于 16S rRNA 基因序列相似性,菌株 P-18 T、JC-4 和 JC-7 分别与Weizmannia coagulans LMG 6326 T (97.27–97.64%) 和W. acidiproducens KCTC 13078 T (96.46–96.74%) 密切相关. 它们含有中- 细胞壁肽聚糖中的二氨基庚二酸,具有七个异戊二烯单元 (MK-7) 作为主要的甲萘醌。菌株PP-18 T的主要细胞脂肪酸是iso -C 15:0、anteiso-C 17:0、iso-C 16:0和anteiso-C 15:0。菌株PP-18 T、JC-4和JC-7基因组间的ANIb和ANIm值均在99.4%以上,而它们与W. coagulans LMG 6326 TW. acidiproducens KCTC 13078 T的ANIb和ANIm值在范围内从 76.61 到 79.59%。这 3 株菌株的数字 DNA-DNA 杂交 (dDDH) 值为 20.7–23.6%W. coagulans LMG 6326 T和 W. acidiproducens DSM 23148 T。菌株PP-18T、JC-4和JC-7的DNA G +C含量分别为45.82%、45.86%和45.86%。主要的极性脂质是二磷脂酰甘油、磷脂酰甘油和磷酸糖脂。表型和化学分类学特征以及全基因组分析的结果表明,菌株 PP-18 T、JC-4 和 JC-7 应该代表魏茨曼属中的一个新物种,其名称为Weizmannia acidilactici sp。十一月 被提议。类型菌株是 PP-18 T (=KCTC 33974 T  = NBRC 113028 T = TISTR 2515 T)。

更新日期:2022-12-14
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