Lignin is one of the most versatile and complex macromolecules, which can be converted to value-added products such as p-coumaric acid and vanillin upon depolymerization. The current work explored oxidative lignin depolymerization in a microbial peroxide-producing cell containing manganese peroxidase enzymes. A double-chambered microbial peroxide-producing cell was constructed containing the immobilized manganese peroxidase on alginate beads in the cathode chamber, while the anodic chamber contained wastewater. This setup was run for 8 days after the addition of lignin in the catholyte. The voltage measured in the circuit was 0.491 V while the current and power densities were 223 µA/cm² and 110 µW/cm², respectively on the 8th day of the experiment. The maximum H₂O₂ concentration observed was 1.5 mM on the 6th day. Depolymerization of lignin was confirmed by the change in the significant peaks at 280 nm of the ultraviolet-visible spectrum. A change in the signature regions of β-β linkages and β-O-4 linkages in the Fourier-transform infrared spectrum was also observed. Liquid chromatography–quadrupole time of flight–mass spectrometry analysis revealed the presence of compounds including isoeugenol, acetovanillone, methacrylic acid, phenamacril, diofenolan, and jasmolin identified as the product of lignin depolymerization.

中文翻译：

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用于木质素生物精炼的微生物过氧化物产生细胞耦合原位酶促解聚

木质素是用途最广、最复杂的大分子之一，解聚后可转化为对香豆酸、香兰素等高附加值产品。目前的工作探索了含有锰过氧化物酶的微生物过氧化物产生细胞中的氧化木质素解聚。构建了一个双室微生物过氧化物生产电池，在阴极室中包含固定在藻酸盐珠上的锰过氧化物酶，而阳极室则装有废水。在阴极电解液中添加木质素后，此设置运行 8 天。在实验的第 8 天，电路中测得的电压为 0.491 V，而电流和功率密度分别为 223 µA/cm ²和 110 µW/cm ^{2 。}最大H ₂在第 6 天观察到的O _{2浓度为 1.5 mM。}通过紫外-可见光谱 280 nm 处的显着峰的变化证实了木质素的解聚。还观察到傅立叶变换红外光谱中 β-β 连接和 β-O-4 连接的特征区域的变化。液相色谱-四极杆飞行时间-质谱分析显示存在化合物，包括异丁香酚、乙酰香草酮、甲基丙烯酸、苯甲腈、二苯酚醇和茉莉花素，这些化合物被确定为木质素解聚的产物。