Abstract

The effects of blueberry anthocyanins against perfluorooctane sulfonate on antioxidant-related gene expression levels, antioxidase activities, malondialdehyde content, glutathione/oxidized glutathione ratios, mitochondrial metabolic enzyme activities, and mitochondrial permeability transition pore opening rates were examined in the planarian Dugesia japonica. Glutathione reductase, glutathione peroxidase, and glutathione S-transferase gene expression levels were up-regulated on day 1 and then down-regulated following exposure to 5 mg/L perfluorooctane sulfonate with those of the control. The activities of the three corresponding enzymes were inhibited by perfluorooctane sulfonate and activated by anthocyanins. Malondialdehyde contents and mitochondrial permeability transition pore opening rates as well as the activities of mitochondrial metabolic enzymes, also increased and the glutathione/oxidized glutathione ratio decreased. Anthocyanin treatments have an alleviating effect on the disorder caused by perfluorooctane sulfonate.

摘要

研究了蓝莓花青素对全氟辛烷磺酸对涡虫抗氧化相关基因表达水平、抗氧化酶活性、丙二醛含量、谷胱甘肽/氧化型谷胱甘肽比值、线粒体代谢酶活性和线粒体通透性转换孔开放率的影响。。与对照组相比，谷胱甘肽还原酶、谷胱甘肽过氧化物酶和谷胱甘肽 S-转移酶基因表达水平在第 1 天上调，然后在暴露于 5 mg/L 全氟辛烷磺酸后下调。三种相应酶的活性被全氟辛烷磺酸抑制并被花青素激活。丙二醛含量和线粒体通透性转换孔开放率以及线粒体代谢酶的活性也增加，谷胱甘肽/氧化型谷胱甘肽比值降低。花青素治疗对全氟辛烷磺酸引起的疾病有缓解作用。