The human cytomegalovirus (HCMV) is an asymptomatic common virus that is typically harmless, but in some cases, it can be life threatening. Thus, there is an urgent need to develop novel diagnostic methods and strengthen the efforts to combat this virus. A microcantilever-based biosensor functionalised with the UL83-antibody of HCMV (UL83-HCMV antibody) has been developed to detect the UL83-antigen of HCMV (UL83-HCMV antigen) at different concentrations ranging from 0.3 to 300 ng/ml. The response of the biosensor to the presence of UL83-HCMV antigen was measured through the shift in resonance frequency before and after antigen-antibody binding. The system shows a low detection limit of 84 pg/ml, which is comparable to traditional sensors, and a detection time of less than 15 min was achieved. The selectivity of the sensor was demonstrated using three different proteins with and without the UL83-HCMV antigen. The biosensor shows high selectivity for the UL83-HCMV antigen. Mass loading by the UL83-HCMV antigen was roughly estimated with a sensitivity of ∼30 fg/Hz. This technique is crucial for the fabrication of portable and low-cost biosensors that can be used in real-time monitoring and enables early medical diagnosis.

中文翻译：

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基于微机电系统的生物传感器，用于人巨细胞病毒的无标记检测。

人巨细胞病毒 (HCMV) 是一种无症状的常见病毒，通常无害，但在某些情况下，它可能会危及生命。因此，迫切需要开发新的诊断方法并加强与这种病毒的斗争。开发了一种基于微悬臂梁的生物传感器，其功能化为 HCMV 的 UL83 抗体（UL83-HCMV 抗体），用于检测 0.3 至 300 ng/ml 的不同浓度的 HCMV 的 UL83 抗原（UL83-HCMV 抗原）。生物传感器对 UL83-HCMV 抗原存在的反应是通过抗原抗体结合前后共振频率的变化来测量的。该系统显示出 84 pg/ml 的低检测限，与传统传感器相当，检测时间不到 15 分钟。使用具有和不具有 UL83-HCMV 抗原的三种不同蛋白质证明了传感器的选择性。该生物传感器对 UL83-HCMV 抗原显示出高选择性。粗略估计 UL83-HCMV 抗原的质量负载，灵敏度为 ~30 fg/Hz。该技术对于制造可用于实时监测和早期医学诊断的便携式低成本生物传感器至关重要。