Bioremediation of polycyclic aromatic hydrocarbons (PAHs) in contaminated soils are reported in many literatures. Composting, in addition to bioremediation, can simultaneously increase soil organic matter content and soil fertility and is thus regarded as one of the most cost-effective methods of soil remediation. In this study, biodegradation of phenanthrene (PHE) and pyrene (PYR) is reported by microbial consortia enriched by vermicompost. After soil samples preparation and grinding, the samples were contaminated with 100, 200, and 300 mg/kg of PHE and PYR concentrations and inoculated with three concentrations (2, 4, and 6 wt.%) of vermicompost. PHE and PYR concentrations were analyzed by HPLC during bioremediation. After 70 days, two highly capable microbial consortia were used to remove the pollutants in bioaugmentation conditions. Analysis of their microbial composition revealed that the consortia contain several Proteobacteria phylum bacterial species, and the most common genera were Pseudomonas and Citrobacter. Decontamination rates for PHE and PYR were estimated to be 89% and 83% over 45 days, respectively. Biodegradation kinetics revealed that microbial degradation followed a first-order kinetics. This study provides clear evidence on the biodegradation of PHE and PYR, paving the way for the development of bioremediation technologies for the recovery of polluted ecosystems.

中文翻译：

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蚯蚓粪对污染土壤中多环芳烃的生物修复

许多文献都报道了污染土壤中多环芳烃 (PAH) 的生物修复。堆肥除生物修复外，还可以同时增加土壤有机质含量和土壤肥力，因此被认为是最具成本效益的土壤修复方法之一。在这项研究中，通过蚯蚓粪富集的微生物群落报告了菲 (PHE) 和芘 (PYR) 的生物降解。土壤样品制备和研磨后，样品被浓度为 100、200 和 300 mg/kg 的 PHE 和 PYR 污染，并接种三种浓度（2、4 和 6 wt.%）的蚯蚓粪。在生物修复过程中，通过 HPLC 分析 PHE 和 PYR 浓度。70 天后，在生物强化条件下，两个高效微生物群被用于去除污染物。假单胞菌和柠檬酸杆菌。PHE 和 PYR 在 45 天内的去污率估计分别为 89% 和 83%。生物降解动力学表明微生物降解遵循一级动力学。这项研究为 PHE 和 PYR 的生物降解提供了明确的证据，为开发用于恢复受污染生态系统的生物修复技术铺平了道路。