Background: The association between high-risk serotypes of human papillomavirus (hr-HPV) and cervical cancer is well-established. Summary: In order to improve the sensitivity of cervical cytology testing, hr-HPV testing has rapidly become part of routine cervical cancer screening, either in conjunction with cytology or as primary testing. In this review, we discuss the overall utility and strategies of hr-HPV testing, as well as the advantages and limitations of potential triage strategies for hr-HPV positive women, including HPV genotyping, p16/Ki-67 dual staining, and methylation assays. Key Message: hr-HPV testing is discussed as primary screening and HPV genotyping, p16/Ki-67 dual staining, and methylation assays are discussed as ancillary techniques to cytology in the triage of hr-HPV positive women undergoing cervical cancer screening.


中文翻译：

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宫颈细胞学辅助技术概述

背景：人乳头瘤病毒 (hr-HPV) 高危血清型与宫颈癌之间的关联已得到确认。总结：为了提高宫颈细胞学检测的敏感性，hr-HPV 检测已迅速成为常规宫颈癌筛查的一部分，与细胞学结合或作为主要检测。在这篇综述中，我们讨论了 hr-HPV 检测的总体效用和策略，以及 hr-HPV 阳性女性潜在分诊策略的优势和局限性，包括 HPV 基因分型、p16/Ki-67 双染色和甲基化检测. 关键信息：hr-HPV 检测被讨论为初级筛查，HPV 基因分型、p16/Ki-67 双染色和甲基化检测被讨论为对接受宫颈癌筛查的 hr-HPV 阳性女性进行细胞学分流的辅助技术。