Introduction: Cervical cancer (CC) is a prevailing malignant tumor in women, mainly caused by human papillomavirus (HPV) infection. This study investigated miR-106a expression in the serum of HPV-positive CC patients and estimated its value in diagnosis and prognosis. Methods: We enrolled 120 CC patients as study subjects, with another 80 healthy women as controls. Clinical baseline data and clinicopathological indexes including age, tumor size, differentiation degree, FIGO stage, lymph node metastasis, and SCC-Ag were recorded. Serum miR-106a expression was measured using RT-qPCR. ROC curve was employed to estimate the efficacy of miR-106a in diagnosing CC or HPV-positive CC. Under a 5-year follow-up, patient survival was recorded, and the impact of miR-106a on overall survival rate was analyzed by the Kaplan-Meier method. The logistic regression model was used to analyze whether miR-106a was an independent prognostic factor for HPV infection in CC patients. Results: Serum miR-106a was upregulated in CC patients and the level > 1.365 assisted the CC diagnosis. miR-106a expression in HPV-positive CC patients was elevated relative to HPV-negative CC patients, and serum miR-106a level > 1.300 distinguishing HPV-positive and HPV-negative. HPV positivity was linked with tumor differentiation degree, FIGO stage, lymph node metastasis, and SCC-Ag in CC patients, but not with age and tumor size. High expression of miR-106a in HPV-positive CC patients increased the risk of poor prognosis, and miR-106a expression is an independent prognostic factor for HPV infection in CC patients. Conclusion: High expression of miR-106a assists the diagnosis of HPV-positive CC and predicts poor prognosis.


中文翻译：

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血清miR-106a对人乳头瘤病毒阳性宫颈癌诊断及预后的临床价值

简介：宫颈癌（CC）是女性常见的恶性肿瘤，主要由人乳头瘤病毒（HPV）感染引起。本研究调查了 miR-106a 在 HPV 阳性 CC 患者血清中的表达，并评估了其在诊断和预后中的价值。方法：我们招募了 120 名 CC 患者作为研究对象，另外 80 名健康女性作为对照。记录临床基线资料和临床病理指标，包括年龄、肿瘤大小、分化程度、FIGO分期、淋巴结转移和SCC-Ag。使用 RT-qPCR 测量血清 miR-106a 表达。ROC 曲线用于评估 miR-106a 在诊断 CC 或 HPV 阳性 CC 中的功效。在 5 年的随访中，记录患者的生存情况，并通过 Kaplan-Meier 方法分析 miR-106a 对总体生存率的影响。使用逻辑回归模型分析miR-106a是否是CC患者HPV感染的独立预后因素。结果：血清 miR-106a 在 CC 患者中上调，水平 > 1.365 有助于 CC 诊断。相对于 HPV 阴性 CC 患者，HPV 阳性 CC 患者的 miR-106a 表达升高，血清 miR-106a 水平 > 1.300 可区分 HPV 阳性和 HPV 阴性。HPV 阳性与 CC 患者的肿瘤分化程度、FIGO 分期、淋巴结转移和 SCC-Ag 相关，但与年龄和肿瘤大小无关。HPV阳性CC患者miR-106a的高表达增加了预后不良的风险，miR-106a表达是CC患者HPV感染的独立预后因素。结论：