To enable the accurate reproduction of organs in vitro, and improve drug screening efficiency and regenerative medicine research, it is necessary to assemble cells with single-cell resolution to form cell clusters. However, a method to assemble such forms has not been developed. In this study, a platform for on-site cell assembly at the single-cell level using optically driven microtools in a microfluidic device is developed. The microtool was fabricated by SU-8 photolithography, and antibodies were immobilised on its surface. The cells were captured by the microtool through the bindings between the antibodies on the microtool and the antigens on the cell membrane. Transmembrane proteins, CD51/61 and CD44 that facilitate cell adhesion, commonly found on the surface of cancer cells were targeted. The microtool containing antibodies for CD51/61 and CD44 proteins was manipulated using optical tweezers to capture HeLa cells placed on a microfluidic device. A comparison of the adhesion rates of different surface treatments showed the superiority of the antibody-immobilised microtool. The assembly of multiple cells into a cluster by repeating the cell capture process is further demonstrated. The geometry and surface function of the microtool can be modified according to the cell assembly requirements. The platform can be used in regenerative medicine and drug screening to produce cell clusters that closely resemble tissues and organs in vivo.

中文翻译：

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具有抗体固定化表面的光学驱动微型工具，用于现场细胞组装。

为了能够在体外准确复制器官，提高药物筛选效率和再生医学研究，需要将具有单细胞分辨率的细胞组装成细胞团。但是，尚未开发出组装此类表格的方法。在这项研究中，开发了一个在微流体装置中使用光学驱动的微型工具在单细胞水平上进行现场细胞组装的平台。该微型工具由 SU-8 光刻技术制成，抗体固定在其表面。通过微型工具上的抗体与细胞膜上的抗原之间的结合，微型工具捕获细胞。目标是促进细胞粘附的跨膜蛋白 CD51/61 和 CD44，这些蛋白通常存在于癌细胞表面。使用光学镊子操作包含 CD51/61 和 CD44 蛋白抗体的微型工具，以捕获放置在微流体装置上的 HeLa 细胞。不同表面处理的粘附率比较显示了抗体固定化微型工具的优越性。进一步证明了通过重复细胞捕获过程将多个细胞组装成一个簇。microtool 的几何形状和表面功能可以根据电池组装要求进行修改。该平台可用于再生医学和药物筛选，以产生与体内组织和器官非常相似的细胞簇。不同表面处理的粘附率比较显示了抗体固定化微型工具的优越性。进一步证明了通过重复细胞捕获过程将多个细胞组装成一个簇。microtool 的几何形状和表面功能可以根据电池组装要求进行修改。该平台可用于再生医学和药物筛选，以产生与体内组织和器官非常相似的细胞簇。不同表面处理的粘附率比较显示了抗体固定化微型工具的优越性。进一步证明了通过重复细胞捕获过程将多个细胞组装成一个簇。microtool 的几何形状和表面功能可以根据电池组装要求进行修改。该平台可用于再生医学和药物筛选，以产生与体内组织和器官非常相似的细胞簇。