This study investigated a SARS-CoV-2 virus detection mechanism using dry-stored disposable strips. The accuracy of this sensing platform is as good as polymerase chain reaction (PCR) with a detection time of fewer than 30 s. SARS-CoV-2 antibodies were biofunctionalized on disposable strips similar to glucose detection strips to detect the presence and concentrations of SARS-CoV-2 in saliva samples. Eight 1 ms electric pulses were sent through the sensor strip with a saliva sample in its microfluidic chamber. A circuit board embedded with MOSFET was also employed to amplify the detected signals and convert the signal to digital readings displayed on an LCD screen. The COV-antibody functionalized disposable strips were stored in a dry condition for at least one day before analyzing clinical human saliva samples with known cycling threshold (Ct) values confirmed with conventional PCR tests. Results demonstrate our system is capable of showing qualitative positive or negative results within 30 s and providing quantitative SARS-CoV-2 concentrations in terms of Ct values in 5 min.

中文翻译：

---

在快速 SARS-CoV-2 检测平台上评估干燥储存的一次性传感器条

本研究使用干燥储存的一次性条带调查了 SARS-CoV-2 病毒检测机制。该传感平台的准确性与聚合酶链反应 (PCR) 一样好，检测时间小于 30 秒。SARS-CoV-2 抗体在类似于葡萄糖检测条的一次性条上进行生物功能化，以检测唾液样本中 SARS-CoV-2 的存在和浓度。八个 1 毫秒的电脉冲通过带有唾液样本的传感带发送到其微流体室中。还采用嵌入 MOSFET 的电路板来放大检测到的信号并将信号转换为数字读数，显示在 LCD 屏幕上。COV 抗体功能化的一次性试纸条在干燥条件下储存至少一天，然后分析具有已知循环阈值 (Ct) 值的临床人类唾液样本，这些样本已通过常规 PCR 测试确认。结果表明，我们的系统能够在 30 秒内显示定性阳性或阴性结果，并在 5 分钟内提供以 Ct 值表示的定量 SARS-CoV-2 浓度。