Two heterotrophic bacteroidetes strains were isolated as satellites from autotrophic enrichments inoculated with samples from hypersaline soda lakes in southwestern Siberia. Strain Z-1702^T is an obligate anaerobic fermentative saccharolytic bacterium from an iron-reducing enrichment culture, while Ca. Cyclonatronum proteinivorum Omega^T is an obligate aerobic proteolytic microorganism from a cyanobacterial enrichment. Cells of isolated bacteria are characterized by highly variable morphology. Both strains are chloride-independent moderate salt-tolerant obligate alkaliphiles and mesophiles. Strain Z-1702^T ferments glucose, maltose, fructose, mannose, sorbose, galactose, cellobiose, N-acetyl-glucosamine and alpha-glucans, including starch, glycogen, dextrin, and pullulan. Strain Omega^T is strictly proteolytic utilizing a range of proteins and peptones. The main polar lipid fatty acid in both strains is iso-C_15:0, while other major components are various C₁₆ and C₁₇ isomers. According to pairwise sequence alignments using BLAST Gracilimonas was the nearest cultured relative to both strains (<90% of 16S rRNA gene sequence identity). Phylogenetic analysis placed strain Z-1702^T and strain Omega^T as two different genera in a deep-branching clade of the new family level within the order Balneolales with genus. Based on physiological characteristics and phylogenetic position of strain Z-1702^T it was proposed to represent a novel genus and species Natronogracilivirga saccharolityca gen. nov., sp. nov. (= DSMZ 109061^T =JCM 32930^T =VKM B 3262^T). Furthermore, phylogenetic and phenotypic parameters of N. saccharolityca and C. proteinivorum gen. nov., sp. nov., strain Omega^T (=JCM 31662^T, =UNIQEM U979^T), make it possible to include them into a new family with a proposed designation Cyclonatronaceae fam. nov..

从用西伯利亚西南部高盐碱湖样品接种的自养富集物中分离出两种异养拟杆菌菌株作为卫星。菌株 Z-1702 ^T是一种来自铁还原富集培养物的专性厌氧发酵糖分解细菌，而Ca。Cyclonatronum proteinivorum Omega ^T是一种来自蓝藻富集的专性好氧蛋白水解微生物。分离细菌的细胞以高度可变的形态为特征。两种菌株都是不依赖氯化物的中度耐盐性专性嗜碱菌和嗜温菌。菌株 Z-1702 ^T发酵葡萄糖、麦芽糖、果糖、甘露糖、山梨糖、半乳糖、纤维二糖、N-乙酰氨基葡萄糖和α-葡聚糖，包括淀粉、糖原、糊精和支链淀粉。Strain Omega ^T是严格蛋白水解的，利用一系列蛋白质和蛋白胨。两株中主要的极性脂质脂肪酸是iso-C _15:0，而其他主要成分是各种C ₁₆和C ₁₇异构体。根据使用 BLAST 的成对序列比对，Gracilimonas是最接近两种菌株的培养物（<90% 的 16S rRNA 基因序列同一性）。系统发育分析将菌株 Z-1702 ^T和菌株 Omega ^T作为两个不同的属，置于Balneolales目新科水平的深分支进化枝中与属。基于菌株 Z-1702 ^T的生理特征和系统发育位置，提出代表一个新的属和种Natronogracilivirga saccharolityca gen。十一月，sp。十一月 （= DSMZ 109061 ^T =JCM 32930 ^T =VKM B 3262 ^T）。此外，N. saccharolityca和C. proteinivorum gen 的系统发育和表型参数。十一月，sp。nov., strain Omega ^T (=JCM 31662 ^T , =UNIQEM U979 ^T ), 使得有可能将它们包含在一个新的家族中，并建议命名为Cyclonatronaceae fam。十一月..