In-vitro chemical characterization and mosquitocidal screening of essential oil derived from Mikania scandens (L.) Willd. (Asteraceae) (CVO-MS) against the malarial vector Anopheles gambiae was investigated. GC-MS analysis evidenced a total of 12 bio-active compounds, and maximum at α-bisabolol (39.34%) followed by stigmasterol (13.45%) respectively. The larvicidal activity of CVO-MS against the malarial vector An. gambiae evidenced that the mortality rate was prominent at the maximum dosage of 1000 ppm. The Median Lethal Concentration (LC₅₀) of CVO-MS was established at 488 ± 2.45 ppm, respectively. Enzyme inhibition assay showed that the CVO-MS delivers a significant upsurge in the level of CYP450, GST, and a decline in the level of α-β carboxylesterase activity in both the third and fourth instar (p ≤ 0.0001). The gut-histological examination of CVO-MS showed that there is severe damage in the mid-gut tissues, especially epithelial layer (EL), gut-lumen (GL), and peritrophic membrane (pM). The non-target screening against the mosquito predators (A. bouvieri, D. indicus, and third instar larvae of Tx. splendens) suggests that CVO-MS showed less toxicity as compared to Pestanal® (1 ppm) respectively. The present study has paved a new insight in understanding the bioactivity of CVO-MS as a potential larvicidal agent of malarial vector and non-toxic against mosquito predators.

来自薇甘菊(L.) Willd的精油的体外化学表征和杀蚊筛选。对疟疾载体冈比亚按蚊进行了研究（菊科）(CVO-MS) 。GC-MS 分析证明共有 12 种生物活性化合物，α-红没药醇 (39.34%) 含量最高，其次是豆甾醇 (13.45%)。CVO-MS 对疟疾载体An 的杀幼虫活性。冈比亚证明，在 1000 ppm 的最大剂量下，死亡率非常显着。半数致死浓度 (LC ₅₀) 的 CVO-MS 分别确定为 488 ± 2.45 ppm。酶抑制试验表明，CVO-MS 显着提高了第三龄和第四龄的 CYP450、GST 水平，并降低了 α-β 羧酸酯酶活性水平 ( p ≤  0.0001)。CVO-MS 的肠道组织学检查表明，中肠组织存在严重损伤，尤其是上皮层 (EL)、肠腔 (GL) 和围食膜 (pM)。蚊子捕食者（ A. bouvieri , D. indicus , and third restar larvae of Tx. splendens的非目标筛选) 表明，与 Pestanal® (1 ppm) 相比，CVO-MS 的毒性更低。本研究为理解 CVO-MS 的生物活性铺平了新的思路，作​​为一种潜在的疟疾媒介杀幼虫剂和对蚊子捕食者无毒。