Background: The white-backed planthopper (WBPH), Sogatella furcifera, causes great damage to many crops (mainly rice) by direct feeding or transmitting plant viruses. The previous genome assembly was generated by second-generation sequencing technologies, with a contig N50 of only 51.5 kb, and contained a lot of heterozygous sequences. Methods: We utilized third-generation sequencing technologies and Hi-C data to generate a highquality chromosome-level assembly. We also provide a large amount of transcriptome data for fulllength transcriptome analysis and gender differential expression analysis. Results: The final assembly comprised 56.38 Mb, with a contig N50 of 2.20 Mb and a scaffold N50 of 45.25 Mb. Fourteen autosomes and one X chromosome were identified. More than 99.5% of the assembled bases located on the 15 chromosomes. 95.9% of the complete BUSCO Hemiptera genes were detected in the final assembly and 16,880 genes were annotated. 722 genes were relatively highly expressed in males, while 60 in the females. Conclusion: The integrated genome, definite sex chromosomes, comprehensive transcriptome profiles, high efficiency of RNA interference and short life cycle substantially made WBPH an efficient research object for functional genomics.

中文翻译：

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白背飞虱染色体水平基因组组装和性别特异性差异转录组

背景：白背飞虱（WBPH）通过直接取食或传播植物病毒，对许多农作物（主要是水稻）造成巨大危害。之前的基因组组装是通过二代测序技术生成的，contig N50只有51.5 kb，并且包含大量杂合序列。方法：我们利用第三代测序技术和 Hi-C 数据生成高质量的染色体水平组装。我们还提供大量转录组数据用于全长转录组分析和性别差异表达分析。结果：最终组装包含 56.38 Mb，其中重叠群 N50 为 2.20 Mb，支架 N50 为 45.25 Mb。鉴定出 14 条常染色体和 1 条 X 染色体。超过99.5%的组装碱基位于15条染色体上。最终组装中检测到 95.9% 的完整 BUSCO 半翅目基因，并注释了 16,880 个基因。722个基因在男性中表达相对较高，而60个基因在女性中表达相对较高。结论：WBPH基因组完整、性染色体明确、转录组谱全面、RNA干扰效率高、生命周期短，使其成为功能基因组学的有效研究对象。