Background: Sepsis is an uncontrolled systemic inflammatory response. Long noncoding RNAs (lncRNAs) are involved in the pathogenesis of sepsis. However, little is known about the roles of lncRNAs in sepsis-induced myocardial dysfunction. Objective: We aimed to determine the regulatory mechanism of lncRNAs in sepsis-induced myocardial dysfunction. Methods: In this study, we analysed the lncRNA and mRNA expression profiles using microarray analysis. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, protein-protein interaction network, and gene set enrichment analysis were used to evaluate the data. We also constructed coding and noncoding coexpression and competing endogenous RNA networks to investigate the mechanisms. Results: In vivo lipopolysaccharide -induced sepsis rat model was established. A total of 387 lncRNAs and 1,952 mRNAs were identified as significantly changed in the left ventricle. Kyoto Encyclopedia of Genes and Genomes analysis of mRNAs showed that the upregulated genes were mainly enriched in the “complement and coagulation cascade pathway” and “immune-related biological processes” terms. Eight significantly changed lncRNAs detected by RT-qPCR may be responsible for these processes. A competing endogenous RNA network was generated, and the results indicated that eight lncRNAs were related to the “calcium ion binding” process. Conclusion: These results demonstrate that crosstalk between lncRNAs and mRNAs may play important roles in the development of sepsis-induced myocardial dysfunction.

中文翻译：

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LPS 诱导的心肌功能障碍大鼠的长非编码 RNA 和 mRNA 表达谱

背景：脓毒症是一种不受控制的全身炎症反应。长非编码 RNA (lncRNA) 参与脓毒症的发病机制。然而，人们对 lncRNA 在脓毒症引起的心肌功能障碍中的作用知之甚少。目的：我们的目的是确定 lncRNA 在脓毒症引起的心肌功能障碍中的调节机制。方法：在本研究中，我们使用微阵列分析来分析 lncRNA 和 mRNA 表达谱。使用基因本体论、京都基因和基因组百科全书、蛋白质-蛋白质相互作用网络和基因集富集分析来评估数据。我们还构建了编码和非编码共表达以及竞争性内源 RNA 网络来研究其机制。结果：建立体内脂多糖诱导脓毒症大鼠模型。总共 387 个 lncRNA 和 1,952 个 mRNA 被鉴定为左心室中发生显着变化。京都基因和基因组百科全书的mRNA分析表明，上调的基因主要富集在“补体和凝血级联途径”和“免疫相关生物过程”术语中。RT-qPCR 检测到的 8 个显着变化的 lncRNA 可能与这些过程有关。生成了竞争性内源RNA网络，结果表明8个lncRNA与“钙离子结合”过程相关。结论：这些结果表明，lncRNA 和 mRNA 之间的串扰可能在脓毒症引起的心肌功能障碍的发展中发挥重要作用。