Inland shrimp aquaculture has increased in popularity in recent years as a means of providing fresh seafood to consumers away from the coasts. With adequate marketing,fresh shrimp fetch relatively high prices and can give farmers a means of diversifying, production. In temperate regions such as Kentucky, low-cost high tunnel greenhouses may be used to extend the growing season of warm-weather crops, including tropical animals such as penaeid shrimp. To optimize investments, inland producers are constantly exploring methods to increase overall production. A 100 day-long study examined the effects of stocking density and artificial substrates on water quality, stable isotope dynamics, and production of Litopenaeus vannamei using biofloc-based aquaculture systems in greenhouses. Four treatments were compared: High Density Substrate (HD/S), High Density No-Substrate (HD/NS), Low, Density Substrate (LD/S), and Low Density No-Substrate (LD/NS). Four high tunnel greenhouses each contained four 10.9 m³ tanks. The experiment was blocked by location such that one replicate from each treatment was randomly assigned within each greenhouse to account for potential environmental variation. Each tank was outfitted with a settling chamber for solids filtration, and 13.1 m² of polypropylene, construction safety fencing was used as substrate in the substrate-containing tanks. Shrimp were stocked into the experimental systems at 2.71 g mean weight; high density treatments were stocked with 300 shrimp/m³, while low density treatments were stocked with 200 shrimp/m³. Water temperatures were significantly lower over the course of the entire study within substrate treatments. pH throughout the study was significantly lower within substrate treatments and high density treatments. Stable isotope data indicated that very little of the carbon and nitrogen in shrimp tissues was likely sourced from biofloc and periphyton material. Low density treatments had significantly lower FCRs than high density treatments. However, the addition of substrate in the HD/S treatment resulted in significantly more efficient shrimp FCR than was observed in the HD/NS treatment. The HD/S treatment also had significantly higher, shrimp biomass than all other treatments. High density treatments had significantly higher FCR and lower survival than low density treatments. This study demonstrates that artificial substrates can reduce some of the negative effects of high shrimp stocking density, with significant improvements in FCR and biomass production. Although it is important to note the implications for water quality, adding substrate to facilitate high density shrimp culture in high tunnel greenhouses can facilitate higher stocking density without compromising the size of the animals

作为向远离海岸的消费者提供新鲜海产品的一种方式，内陆对虾水产养殖近年来越来越受欢迎。通过适当的营销，鲜虾可以卖到相对较高的价格，并且可以为农民提供多样化生产的手段。在肯塔基州等温带地区，低成本的高架隧道温室可用于延长暖季作物的生长季节，包括对虾等热带动物。为了优化投资，内陆生产商不断探索提高整体产量的方法。一项为期 100 天的研究考察了放养密度和人工底物对水质、稳定同位素动力学和凡纳滨对虾产量的影响在温室中使用基于生物絮团的水产养殖系统。比较了四种处理：高密度底物 (HD/S)、高密度无底物 (HD/NS)、低密度底物 (LD/S) 和低密度无底物 (LD/NS)。四个高隧道温室各包含四个 10.9 m ³的水箱。实验被地点限制，因此每个处理的一个重复在每个温室内随机分配，以解释潜在的环境变化。每个罐配备有用于固体过滤的沉降室，并且使用13.1m ²的聚丙烯、建筑安全栅栏作为包含底物的罐中的底物。将虾以 2.71 g 的平均重量放养到实验系统中；高密度处理放养 300 虾/m ³, 而低密度处理放养 200 虾/m ³. 在基质处理的整个研究过程中，水温显着降低。在整个研究过程中，底物处理和高密度处理的 pH 值显着降低。稳定同位素数据表明，虾组织中的碳和氮极少可能来自生物絮团和附生生物材料。低密度处理的 FCR 明显低于高密度处理。然而，在 HD/S 处理中添加底物导致比在 HD/NS 处理中观察到的虾 FCR 显着更有效。HD/S 处理的虾生物量也明显高于所有其他处理。与低密度治疗相比，高密度治疗具有显着更高的 FCR 和更低的存活率。这项研究表明，人工基质可以减少高虾放养密度的一些负面影响，显着提高 FCR 和生物量生产。尽管注意对水质的影响很重要，但在高隧道温室中添加基质以促进高密度对虾养殖可以在不影响动物体型的情况下促进更高的放养密度