The problem of aflatoxin contamination emerged gradually in the field of food safety. Surface-enhanced Raman spectroscopy (SERS) is an ultra-sensitive and non-destructive spectroscopy technology with extensive application prospects in the detection field. In this paper, with the detection of AFB1 as the target, Au@Ag NPs substrate with uniform morphology and strong SERS effect was prepared. Furthermore, the intermediates formed by hydrogen bonding between AFB1 and melamine facilitate the binding of toxin molecules to the substrate. Moreover, the AFB1-melamine-Au@Ag NPs detection system was established by optimizing the melamine dosage, and the limit of detection can reach 10-8 mol/L (M). In this method, AFB1 (concentration range of 10-4 M - 10-7 M) in tea oil, the Raman signal intensity of AFB1 shows an excellent linear, logarithmic relationship, and the correlation coefficient is 0.9685. Therefore, this work has achieved simple, sensitive, and stable AFB1 detection and has broad application potential in food safety detection.

中文翻译：

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优化合成 Au@Ag 纳米粒子作为等离子体放大器，通过物体-结合剂-金属 SERS 方法检测痕量 AFB1。

黄曲霉毒素污染问题逐渐在食品安全领域显现出来。表面增强拉曼光谱(SERS)是一种超灵敏、无损的光谱技术，在检测领域具有广泛的应用前景。本文以检测AFB1为靶标，制备了形貌均匀、SERS效应强的Au@Ag NPs基底。此外，AFB1 和三聚氰胺之间通过氢键形成的中间体有助于毒素分子与底物的结合。此外，通过优化三聚氰胺用量，建立了AFB1-三聚氰胺-Au@Ag NPs检测体系，检测限可达10-8 mol/L(M)。在该方法中，AFB1（浓度范围为10-4 M - 10-7 M）在茶油中，AFB1的拉曼信号强度表现出良好的线性、对数关系，相关系数为0.9685。因此，该工作实现了简单、灵敏、稳定的AFB1检测，在食品安全检测中具有广阔的应用潜力。