Alpinia zerumbet is a medicinal and ornamental plant of the Zingiberaceae family cultivated in many parts of the world. The plant is medicinally important and often misidentified with closely related species. The objective of this work was to establish its DNA-based identity and a protocol for high-fidelity micropropagation to support the medicinal plant industry. PCR amplification and subsequent sequencing of RuBisCO large subunit (rbcL) and Maturase K (matK) gene successfully report its DNA barcode. In vitro shoots were induced the best from rhizome buds on Murashige and Skoog (MS) medium supplemented with 6.0 mg L⁻¹ 6-benzyl amino purine and 30 g L⁻¹ sucrose. Microshoots were rooted well in MS medium amended with 1.5 mg L⁻¹ naphthalene acetic acid. Plantlets were satisfactorily acclimated to soils maintained in a growth chamber and subsequently in the open air for 10–20 days. Analyzing eight random amplified polymorphic DNA and eight inter-simple sequence repeat primers resulted in 53 and 60 distinct bands, respectively. Uniform banding patterns suggest the genetic uniformity of the micropropagated plants. The results offer an accurate identification of A. zerumbet from any plant tissue. The micropropagation protocol would be helpful for economically feasible and environmentally sustainable exploitation of this valuable plant.

Alpinia zerumbet是姜科的一种药用和观赏植物，在世界许多地方都有栽培。这种植物具有重要的药用价值，经常被误认为是密切相关的物种。这项工作的目的是建立其基于 DNA 的身份和高保真微繁殖协议，以支持药用植物行业。RuBisCO 大亚基(rbcL)和成熟酶 K ( matK)基因的 PCR 扩增和后续测序成功地报告了其 DNA 条形码。^{在补充有 6.0 mg L -1} 6-苄基氨基嘌呤和 30 g L ^-1的 Murashige 和 Skoog (MS) 培养基上，从根茎芽中诱导的体外芽最好蔗糖。^{微芽在用 1.5 mg L -1}萘乙酸改良的 MS 培养基中生根良好。小植物令人满意地适应了生长室中保持的土壤，随后在露天环境中适应了 10-20 天。分析八个随机扩增的多态性 DNA 和八个简单序列间重复引物分别产生 53 条和 60 条不同的条带。统一的条带模式表明微繁殖植物的遗传一致性。结果提供了从任何植物组织中准确鉴定A. zerumbet的方法。微繁殖协议将有助于对这种有价值的植物进行经济上可行和环境上可持续的开发。