This paper reports a microfluidic device that allows us to measure the effect of local differentiation stimulation on embryoid bodies for the purpose of elucidating the role of the cell-cell interaction of iPS cells in differentiation induction. We developed the local stimulation device having two chambers separated by thin polydimethyl siloxane (PDMS) membrane with through-holes of the diameter smaller than single cell. Embryoid bodies of iPS cells are formed on the membrane and seal the holes, separating the aqueous environments of two chambers without leakage. This configuration brings localized exposure to chemicals for differentiation induction at the area defined by the holes. In this paper, we demonstrated the function of local stimulation using fluorescent solution.

中文翻译：

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局部化学刺激iPS细胞衍生胚状体分化诱导的微流控装置

本文报道了一种微流体装置，它使我们能够测量局部分化刺激对胚状体的影响，目的是阐明 iPS 细胞的细胞间相互作用在分化诱导中的作用。我们开发了具有两个腔室的局部刺激装置，该腔室由薄聚二甲基硅氧烷 (PDMS) 膜隔开，通孔直径小于单个细胞。iPS 细胞的胚状体在膜上形成并密封孔，将两个腔室的水环境分开而不会泄漏。这种配置使局部暴露于化学物质以在孔定义的区域进行分化诱导。在本文中，我们演示了使用荧光溶液进行局部刺激的功能。