ABSTRACT

Oil Red O (ORO) staining is a commonly used experimental technique to detect lipid content in cells or tissues. Freshly prepared ORO in 60% isopropanol is the most widely used method at present. However, isopropanol is volatile and harmful to the human body. It will also affect the interpretation of the results due to the formation of crystals and non-specific diffuse staining. In this paper, by screening and validation, we report a salicylic acid ethanol solution (containing 50% ethanol, 5%-10% salicylic acid) for the preparation of ORO solution, which has a better staining effect on lipid staining in cells and tissues, with a clean background and short dyeing time. What’s more, this ORO solution is non-toxic, convenient to prepare, and can be stored for a long time. Therefore, it is reliable, easy to operate, and can be widely popularized and applied in laboratories.

摘要

油红 O (ORO) 染色是一种常用的实验技术，用于检测细胞或组织中的脂质含量。在 60% 异丙醇中新鲜制备的 ORO 是目前使用最广泛的方法。但异丙醇易挥发，对人体有害。由于晶体的形成和非特异性弥散染色，它也会影响结果的解释。本文通过筛选和验证，报道了一种用于配制ORO溶液的水杨酸乙醇溶液（含50%乙醇，5%-10%水杨酸），对细胞和组织的脂质染色有较好的染色效果，背景干净，染色时间短。更重要的是，这种ORO溶液无毒，配制方便，可长期保存。因此，它可靠，操作方便，