ABSTRACT

The healing of skin wounds is a highly coordinated multi-step process that occurs after trauma including surgical incisions, thermal burns, and chronic ulcers. In this study, the authors investigated lncRNA FOXD2-AS1 function in adipose mesenchymal exosomes from ADMSCs that were successfully extracted. Highly expressed lncRNA FOXD2-AS1 in ADMSCs-exosomes accelerated HaCaT cell migration and proliferation. LncRNA FOXD2-AS1 negatively targeted miR-185-5p, and miR-185-5p negatively targeted ROCK2. Highly expressed lncRNA FOXD2-AS1 in ADMSCs-exosomes promoted HaCaT cell migration and proliferation via down-regulating miR-185-5p and further up-regulating ROCK2. In conclusion, LncRNA FOXD2-AS1 overexpression in ADMSCs derived exosomes might accelerate HaCaT cell migration and proliferation via modulating the miR-185-5p/ROCK2 axis.

摘要

皮肤伤口的愈合是一个高度协调的多步骤过程，发生在创伤后，包括手术切口、热灼伤和慢性溃疡。在这项研究中，作者研究了 lncRNA FOXD2-AS1 在来自成功提取的 ADMSCs 的脂肪间充质外泌体中的功能。ADMSCs-外泌体中高表达的 lncRNA FOXD2-AS1 加速了 HaCaT 细胞的迁移和增殖。LncRNA FOXD2-AS1 负向靶向 miR-185-5p，而 miR-185-5p 负向靶向 ROCK2。ADMSCs-外泌体中高表达的 lncRNA FOXD2-AS1 通过下调 miR-185-5p 并进一步上调 ROCK2 促进 HaCaT 细胞迁移和增殖。总之，LncRNA FOXD2-AS1 在 ADMSCs 衍生的外泌体中的过表达可能通过调节 miR-185-5p/ROCK2 轴来加速 HaCaT 细胞迁移和增殖。