ABSTRACT

The objective of this research was to produce, characterize and evaluate biological activities of magnetic-polymeric nanoparticles loaded with resveratrol. Magnetic nanoparticles of cobalt ferrite (CoFe₂O₄), polymeric nanoparticles of chitosan (CS) and polyethylene glycol (PEG), and polymeric-magnetic nanoparticles containing resveratrol (RSV) were developed. An analytical method using UV-VIS spectroscopy was developed and validated for the determination of RSV in the nanoparticles. The samples were analyzed by dynamic light scattering (DLS) for size, polydispersity index (PDI) and zeta potential. The encapsulation efficiency was determined by an indirect method. Antioxidants (ABTS and DPPH) and antibacterial (MIC and CBM) activities were evaluated. The average size of the nanoparticles varied between 184.6 and 276.5 nm. The magnetic-polymeric nanoparticles showed high encapsulation efficiency (EE) (98.75%) with a slow and prolonged release rate of 33.49% in 120 h. Furthermore, three months of storage showed less than 10% of RSV degradation. Its antioxidant activity (AA) was 170.55 mmol TE g⁻¹ (by the ABTS method) and 130.60 mmol TE g⁻¹ (by the DPPH method). The resveratrol-loaded magnetic-polymeric nanoparticles inhibited the growth of four tested bacteria. Their MIC values were less than 52.36 µg mL⁻¹, and the CBM values of the nanoparticles >52.36 µg mL⁻¹.

摘要

本研究的目的是生产、表征和评估载有白藜芦醇的磁性聚合物纳米粒子的生物活性。钴铁氧体磁性纳米颗粒（CoFe ₂ O ₄), 开发了壳聚糖 (CS) 和聚乙二醇 (PEG) 的聚合物纳米颗粒，以及含有白藜芦醇 (RSV) 的聚合物磁性纳米颗粒。开发并验证了一种使用紫外-可见光谱的分析方法，用于测定纳米颗粒中的 RSV。通过动态光散射 (DLS) 分析样品的大小、多分散指数 (PDI) 和 zeta 电位。封装效率通过间接方法测定。评估了抗氧化剂（ABTS 和 DPPH）和抗菌（MIC 和 CBM）活性。纳米颗粒的平均尺寸在 184.6 和 276.5 纳米之间变化。磁性聚合物纳米颗粒显示出高封装效率 (EE) (98.75%)，在 120 小时内具有 33.49% 的缓慢和延长释放率。此外，三个月的储存显示 RSV 降解不到 10%。^-1（ABTS法）和130.60 mmol TE g ^-1（DPPH法）。载有白藜芦醇的磁性聚合物纳米颗粒抑制了四种受试细菌的生长。它们的 MIC 值小于 52.36 µg mL ^-1，纳米颗粒的 CBM 值 >52.36 µg mL ^-1。