Ozone (O₃) is not only an environment pollutant at tropospheric or ground-level that poses a potential threat to human health, but it also belongs to the class of reactive oxygen species (ROS) which has been demonstrated to be deeply involved in significant physiological and pharmacological functions. Additionally, this reactive molecule has been widely applied in modern society, for example in air sterilization, and in both food and medical disinfection. Hence, effective detection methods for ozone in both the environment and in biological systems are a necessity. In recent years, the detection systems based on fluorescent strategy have manifested as promising methods for rapid, effective, and quantitative detection, specifically for the spatial and temporal sampling for in vivo bioimaging analysis. Despite a huge surge in the rationally-designed fluorescent probes for specific ROS, relatively limited attention has been paid to the fluorescent recognition of O₃. Particularly, there is no systematic summarization for O₃-specific fluorescent probes. In this review, a general overview was provided with the aim of summarizing the progress made so far in this subject. The chemical structures of the probes, and their sensing mechanisms with O₃ and application in biological and environmental science are discussed in detail. Hopefully, this timely review can give useful information to the researchers in this field, resulting in this emerging area gaining more traction.

臭氧（O ₃)不仅是对流层或地面的环境污染物，对人类健康构成潜在威胁，而且还属于活性氧(ROS)类，已被证明与重要的生理和药理功能密切相关. 此外，这种活性分子在现代社会中得到了广泛的应用，例如空气消毒，以及食品和医疗消毒。因此，有必要对环境和生物系统中的臭氧进行有效检测。近年来，基于荧光策略的检测系统已成为快速、有效和定量检测的有前途的方法，特别是用于体内生物成像分析的空间和时间采样。₃ . 特别是，没有针对O ₃特异性荧光探针的系统总结。在这次审查中，提供了一个总体概述，目的是总结迄今为止在该主题上取得的进展。详细讨论了探针的化学结构、对O _{3 的传感机制以及在生物和环境科学中的应用。}希望这次及时的审查可以为该领域的研究人员提供有用的信息，从而使这一新兴领域获得更多关注。