Abstract

Aims

The aim of this study is to develop a liposome that could exert unparalleled antioxidant effects. In the present study, a vitamin C (VC)/vitamin E (VE)-co-loaded multivesicular liposome (VCVE-MVL) was constructed.

Methods

Vitamins were encapsulated in soybean phosphatidylcholine (SPC) and cholesterol (CHO) by multi-emulsification method. The concentration of VC was determined by Fast Blue method. The concentration of VE was determined by high performance liquid chromatography (HPLC). Vitamin antioxidant capacity in vitro and in vivo was determined using β-carotene bleaching.

Results

VCVE-MVL with particle diameter of 848.55 ± 0.29 nm and SPAN of 0.16 ± 0.11 were obtained. The encapsulation efficiency of VC reached 48.51% (w/w)±0.15. Compared with VC/VE solution, VCVE-MVL had a higher permeation efficiency. In addition, the in vitro and ex-vivo antioxidant tests verified the adequate antioxidant activity of VCVE-MVL.

Conclusions

In conclusion, the antioxidant synergistic system we constructed and demonstrated its potential applications in the cosmetics industry.

中文翻译：

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含维生素C和维生素E的抗氧化协同体系的构建与评价

摘要

宗旨

本研究的目的是开发一种可以发挥无与伦比的抗氧化作用的脂质体。在本研究中，构建了维生素 C (VC)/维生素 E (VE) 共载多泡脂质体 (VCVE-MVL)。

方法

采用多重乳化法将维生素包裹在大豆磷脂酰胆碱（SPC）和胆固醇（CHO）中。VC的浓度用固蓝法测定。VE的浓度通过高效液相色谱法(HPLC)测定。使用 β-胡萝卜素漂白法测定维生素体外和体内的抗氧化能力。

结果

获得了粒径为 848.55 ± 0.29 nm 和 SPAN 为 0.16 ± 0.11 的 VCVE-MVL。VC的包封率达到48.51% (w/w)±0.15。与VC/VE溶液相比，VCVE-MVL具有更高的渗透效率。此外，体外和体外抗氧化试验证实了 VCVE-MVL 具有足够的抗氧化活性。

结论

总之，我们构建并展示了其在化妆品行业的潜在应用的抗氧化协同系统。