Adult zebrafish regenerate their appendages (fins) after amputation including the regeneration of bone structures (fin rays). Fibroblast growth factor (Fgf) signaling, which is involved in morphogenetic processes during development, has been shown to be essential for the process of fin regeneration. Moreover, mutations in Fgf pathway component genes lead to abnormal skeletal growth in teleosts and mammals, including humans, illustrating the importance of Fgf signaling in the growth control of tissues. Here, we revisited Fgf signaling pathway component expression by RNA in situ hybridization to test for the expression of about half of the ligands and all receptors of the pathway in the regenerating zebrafish fin. Expression patterns of fgf7, fgf10b, fgf12b, fgf17b and fgfr1b have not been reported in the literature before. We summarize and discuss known and novel localization of expression and find that all five Fgf receptors (fgfr1a, fgfr1b, fgfr2, fgfr3 and fgfr4) and most of the tested ligands are expressed in specific regions of the regenerate. Our work provides a basis to study domain specific functions of Fgf signaling in the regenerating teleost appendage.

成年斑马鱼在截肢后会再生其附肢（鳍），包括骨结构（鳍条）的再生。成纤维细胞生长因子 (Fgf) 信号传导参与发育过程中的形态发生过程，已被证明对于鳍再生过程至关重要。此外，Fgf 通路组成基因的突变导致硬骨鱼和哺乳动物（包括人类）骨骼生长异常，这说明了 Fgf 信号在组织生长控制中的重要性。在这里，我们通过RNA原位杂交重新审视了Fgf信号通路成分的表达，以测试再生斑马鱼鳍中该通路的大约一半配体和所有受体的表达。fgf7、fgf10b、的表达模式fgf12b、fgf17b和fgfr1b此前未见文献报道。我们总结并讨论了已知和新颖的表达定位，发现所有五种 Fgf 受体（fgfr1a、fgfr1b、fgfr2、fgfr3 和 fgfr4）和大多数测试的配体都在再生的特定区域中表达。我们的工作为研究再生硬骨鱼附肢中 Fgf 信号传导的域特异性功能提供了基础。