Optimal time spans in homograft procurement are still debatable among tissue banks and needs to be further investigated. Cell viability decreases at longer preparation intervals, but the effect on collagen and elastic fibers has not been investigated to the same extent. These fibers are of importance to the homograft elasticity and strength. The objective of this study was to analyze the mechanical properties of homograft tissue at different time spans in the procurement process. Ten aortic homografts were collected at the Tissue Bank in Lund. Twelve samples were obtained from each homograft, cryopreserved in groups of three after 2–4 days, 7–9 days, 28–30 days, and 60–62 days in antibiotic decontamination. Mechanical testing was performed with uniaxial tensile tests, calculating elastic modulus, yield stress and energy at yield stress. Two randomly selected samples were assessed with light microscopy. Procurement generated a total of 120 samples, with 30 samples in each time group. Elastic modulus and yield stress was significantly higher in samples cryopreserved after 2–4 days (2.7 MPa (2.5-5.0) and 0.78 MPa (0.68-1.0)) compared to 7–9 days (2.2 MPa (2.0-2.6) and 0.53 MPa (0.46–0.69)), p = 0.008 and 0.011 respectively. Light microscopy did not show any difference in collagen and elastin at different time spans. There was a significant decrease in elastic modulus and yield stress after 7 days of decontamination at 4 °C compared to 2–4 days. This could indicate some deterioration of elastin and collagen at longer decontamination intervals. Clinical significance of these findings remains to be clarified.

同种移植物采购的最佳时间跨度在组织库中仍有争议，需要进一步研究。较长的制备间隔会降低细胞活力，但对胶原蛋白和弹性纤维的影响尚未进行相同程度的研究。这些纤维对同种移植物的弹性和强度很重要。本研究的目的是分析同种移植组织在采购过程中不同时间跨度的机械特性。在隆德的组织库收集了 10 个主动脉同种移植物。从每个同种移植物中获取 12 个样本，在抗生素去污 2-4 天、7-9 天、28-30 天和 60-62 天后，以三个为一组冷冻保存。机械测试通过单轴拉伸测试进行，计算弹性模量、屈服应力和屈服应力下的能量。用光学显微镜评估两个随机选择的样品。采购共生成120个样本，每个时间组30个样本。与 7-9 天（2.2 MPa（2.0-2.6）和 0.53 MPa）相比，冷冻保存 2-4 天（2.7 MPa（2.5-5.0）和 0.78 MPa（0.68-1.0））的样品的弹性模量和屈服应力显着更高(0.46–0.69)),p  = 0.008 和 0.011 分别。光学显微镜未显示不同时间跨度的胶原蛋白和弹性蛋白有任何差异。与 2-4 天相比，在 4°C 下净化 7 天后，弹性模量和屈服应力显着降低。这可能表明在较长的去污间隔内，弹性蛋白和胶原蛋白会发生一些退化。这些发现的临床意义仍有待阐明。