The purpose of this study is the design and synthesis of gold nanoparticles (GNPs) conjugated with paclitaxel and to investigate the parameters affecting the stability of synthesised nanoparticles with drug delivery capability. Here, synthesised GNPs were coated with polyethylene glycol. Then these particles were conjugated with paclitaxel under different conditions and the physical and structural characteristics, as well as the factors affecting the loading of paclitaxel on nanoparticles, were evaluated by ultraviolet spectrophotometer, fourier transform infrared spectroscopy, transmission electron microscopy, dynamic light scattering and zeta potential apparatus. It was found that pegylated GNPs have a limited loading capacity at the time of 24 h of incubation and the Paclitaxel loading was observed to be pH dependent. The use of these particles in the treatment of breast cancer (MCF7) was also investigated using the MTT test. It was determined that the survival percentage of MCF7 cells in the presence of paclitaxel-bound nanoparticles decreases to about 55% at the maximum measured concentration (690 μM).

中文翻译：

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评估影响抗癌药物紫杉醇负载在用于乳腺癌治疗的包覆金纳米粒子上的参数。

本研究的目的是设计和合成与紫杉醇偶联的金纳米粒子 (GNP)，并研究影响合成纳米粒子稳定性和药物递送能力的参数。在这里，合成的 GNP 涂有聚乙二醇。然后将这些颗粒在不同条件下与紫杉醇缀合，并通过紫外分光光度计、傅立叶变换红外光谱、透射电子显微镜、动态光散射和 zeta 评估物理和结构特征，以及影响紫杉醇负载在纳米颗粒上的因素潜在的设备。发现聚乙二醇化 GNP 在孵育 24 小时时具有有限的负载能力，并且观察到紫杉醇负载具有 pH 依赖性。还使用 MTT 测试研究了这些颗粒在治疗乳腺癌 (MCF7) 中的用途。已确定在最大测量浓度 (690 μM) 下，MCF7 细胞在结合紫杉醇的纳米颗粒存在下的存活百分比降低至约 55%。