Hybrid nanoparticles are innovative invention of last decade designed to overcome limitations of single-component nanoparticles by introducing multiple functionalities through combining two or more different nanoparticles. In this study, we are reporting development of magneto-fluorescent hybrid nanoparticles by combining iron oxide and carbon nanoparticles to enable in vivo fluorescence imaging which also has all the required characteristic properties to use as Magnetic Resonance Imaging (MRI) contrast agent. In order to achieve dual-functional imaging, alginate and pullulan coated super paramagnetic iron oxide nanoparticles (ASPION and PSPION) and Carbon dots (Cdts) were synthesised separately. ASPIONs and PSPIONs were further chemically conjugated with Cdts and developed dual-functional nanohybrid particles ASPION-Cdts and PSPION-Cdts. Subsequently, evaluation of the materials for its size, functionalisation efficiency, fluorescence and magnetic properties, biocompatibility and cellular uptake efficiency has been carried out. Fluorescence imaging of liver fibrosis was performed in vivo in rodent model of liver fibrosis using the two nanohybrids, which is further confirmed by high fluorescence signal from the harvested liver.

中文翻译：

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用于肝纤维化体内光学成像的荧光碳点定制氧化铁纳米杂化系统

杂化纳米颗粒是近十年来的创新发明，旨在通过结合两种或多种不同的纳米颗粒来引入多种功能，从而克服单组分纳米颗粒的局限性。在这项研究中，我们报告了通过结合氧化铁和碳纳米粒子来开发磁荧光混合纳米粒子，以实现体内荧光成像，它还具有用作磁共振成像 (MRI) 造影剂所需的所有特性。为了实现双功能成像，分别合成了海藻酸盐和支链淀粉包裹的超顺磁性氧化铁纳米粒子（ASPION 和 PSPION）和碳点（Cdts）。ASPION 和 PSPION 进一步与 Cdts 发生化学偶联，并开发出双功能纳米杂化粒子 ASPION-Cdts 和 PSPION-Cdts。随后，对材料的尺寸、功能化效率、荧光和磁性、生物相容性和细胞摄取效率进行了评估。进行了肝纤维化的荧光成像体内在使用两种纳米杂化物的肝纤维化啮齿动物模型中，收获的肝脏的高荧光信号进一步证实了这一点。